dc.creator | Murat, C.B. | |
dc.creator | Braga, P.B.S. | |
dc.creator | Fortes, M.A.H.Z. | |
dc.creator | Bronstein, M.D. | |
dc.creator | Corrêa-Giannella, M.L.C. | |
dc.creator | Giorgi, R.R. | |
dc.date.accessioned | 2013-11-04T12:19:08Z | |
dc.date.accessioned | 2018-07-04T16:33:47Z | |
dc.date.available | 2013-11-04T12:19:08Z | |
dc.date.available | 2018-07-04T16:33:47Z | |
dc.date.created | 2013-11-04T12:19:08Z | |
dc.date.issued | 2012 | |
dc.identifier | Braz J Med Biol Res,v.45,n.9,p.851-855,2012 | |
dc.identifier | 0100-879X | |
dc.identifier | http://www.producao.usp.br/handle/BDPI/38787 | |
dc.identifier | 10.1590/S0100-879X2012007500115 | |
dc.identifier | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000900009&lng=en&nrm=iso&tlng=en | |
dc.identifier | http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0100-879X2012000900009&lng=en&nrm=iso&tlng=en | |
dc.identifier | http://www.scielo.br/scielo.php?script=sci_pdf&pid=S0100-879X2012000900009&lng=en&nrm=iso&tlng=en | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1637284 | |
dc.description.abstract | The tumorigenesis of pituitary adenomas is poorly understood. Mutations of the PIK3CA proto-oncogene, which encodes the p110-α catalytic subunit of PI3K, have been reported in various types of human cancers regarding the role of the gene in cell proliferation and survival through activation of the PI3K/Akt signaling pathway. Only one Chinese study described somatic mutations and amplification of the PIK3CA gene in a large series of pituitary adenomas. The aim of the present study was to determine genetic alterations of PIK3CA in a second series that consisted of 33 pituitary adenomas of different subtypes diagnosed by immunohistochemistry: 6 adrenocorticotropic hormone-secreting microadenomas, 5 growth hormone-secreting macroadenomas, 7 prolactin-secreting macroadenomas, and 15 nonfunctioning macroadenomas. Direct sequencing of exons 9 and 20 assessed by qPCR was employed to investigate the presence of mutations and genomic amplification defined as a copy number ≥4. Previously identified PIK3CA mutations (exon 20) were detected in four cases (12.1%). Interestingly, the Chinese study reported mutations only in invasive tumors, while we found a PIK3CA mutation in one noninvasive corticotroph microadenoma. PIK3CA amplification was observed in 21.2% (7/33) of the cases. This study demonstrates the presence of somatic mutations and amplifications of the PIK3CA gene in a second series of pituitary adenomas, corroborating the previously described involvement of the PI3K/Akt signaling pathway in the tumorigenic process of this gland. | |
dc.language | eng | |
dc.publisher | Associação Brasileira de Divulgação Científica | |
dc.relation | Brazilian Journal of Medical and Biological Research | |
dc.rights | openAccess | |
dc.subject | Pituitary adenomas | |
dc.subject | PIK3CA proto-oncogene | |
dc.subject | Genomic amplification | |
dc.subject | Somatic mutation | |
dc.title | Mutation and genomic amplification of the PIK3CA proto-oncogene in pituitary adenomas | |
dc.type | Artículos de revistas | |