Artículos de revistas
Increased expression of miR-221 is associated with shorter overall survival in T-cell acute lymphoid leukemia
Fecha
2013Registro en:
Experimental Hematology & Oncology, London, v.2, 2013
2162-3619
10.1186/2162-3619-2-10
Autor
Teixeira, Hamilton Luiz Gimenes
Araújo, Antonio Roberto Lucena de
Santos, Guilherme A. dos
Zanette, Dalila Lucíola
Scheucher, Priscila S.
Oliveira, Luciana C.
Dalmazzo, Leandro Felipe Figueiredo
Silva Junior, Wilson Araújo da
Falcão, Roberto Passetto
Rego, Eduardo Magalhães
Institución
Resumen
Background: CD56 expression has been associated with a poor prognosis in lymphoid neoplasms, including T-cell acute lymphoblastic leukemia (T-ALL). MicroRNAs (miRNAs) play an important role in lymphoid differentiation, and aberrant miRNA expression has been associated with treatment outcome in lymphoid malignancies. Here, we evaluated miRNA expression profiles in normal thymocytes, mature T-cells, and T-ALL samples with and without CD56 expression and correlated microRNA expression with treatment outcome.
Methods: The gene expression profile of 164 miRNAs were compared for T-ALL/CD56+ (n=12) and T-ALL/CD56- (n=36) patients by Real-Time Quantitative PCR. Based on this analysis, we decided to evaluate miR-221 and miR-374 expression in individual leukemic and normal samples.
Results: miR-221 and miR-374 were expressed at significantly higher levels in T-ALL/CD56+ than in T-ALL/CD56- cells and in leukemic blasts compared with normal thymocytes and peripheral blood (PB) T-cells. Age at diagnosis (15 or less vs grater than 15 years; HR: 2.19, 95% CI: 0.98-4.85; P=0.05), miR-221 expression level (median value as cut
off in leukemic samples; HR: 3.17, 95% CI: 1.45-6.92; P=0.004), and the expression of CD56 (CD56- vs CD56+; HR: 2.99, 95% CI: 1.37-6.51; P=0.006) were predictive factors for shorter overall survival; whereas, only CD56 expression (HR: 2.73, 95% CI: 1.03-7.18; P=0.041) was associated with a shorter disease-free survival rate.
Conclusions: miR-221 is highly expressed in T-ALL and its expression level may be associated with a poorer prognosis.