dc.creatorColombo, Jucimara 
dc.creatorProvazzi, Paola Jocelan 
dc.creatorCalmon, Marilia 
dc.creatorPires, Lilian 
dc.creatorRodrigues, Nathalia Campos
dc.creatorPetl, Paulo 
dc.creatorFossey, Marcelo 
dc.creatorSouza, Fátima de
dc.creatorCanduri, Fernanda
dc.creatorRahal, Paula 
dc.date.accessioned2013-10-14T17:50:28Z
dc.date.accessioned2018-07-04T16:30:13Z
dc.date.available2013-10-14T17:50:28Z
dc.date.available2018-07-04T16:30:13Z
dc.date.created2013-10-14T17:50:28Z
dc.date.issued2013
dc.identifierBiological Procedures Online, London, v.15, n.10, p. 1-8, 2013
dc.identifier1480-9222
dc.identifierhttp://www.producao.usp.br/handle/BDPI/34805
dc.identifier10.1186/1480-9222-15-10
dc.identifierhttp://www.biologicalproceduresonline.com/content/15/1/10
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1636479
dc.description.abstractBackground: The ZNF706 gene encodes a protein that belongs to the zinc finger family of proteins and was found to be highly expressed in laryngeal cancer, making the structure and function of ZNF706 worthy of investigation. In this study, we expressed and purified recombinant human ZNF706 that was suitable for structural analysis in Escherichia coli BL21(DH3). Findings: ZNF706 mRNA was extracted from a larynx tissue sample, and cDNA was ligated into a cloning vector using the TOPO method. ZNF706 protein was expressed according to the E. coli expression system procedures and was purified using a nickel-affinity column. The structural qualities of recombinant ZNF706 and quantification alpha, beta sheet, and other structures were obtained by spectroscopy of circular dichroism. ZNF706's structural modeling showed that it is composed of α-helices (28.3%), β-strands (19.4%), and turns (20.9%), in agreement with the spectral data from the dichroism analysis. Conclusions: We used circular dichroism and molecular modeling to examine the structure of ZNF706. The results suggest that human recombinant ZNF706 keeps its secondary structures and is appropriate for functional and structural studies. The method of expressing ZNF706 protein used in this study can be used to direct various functional and structural studies that will contribute to the understanding of its function as well as its relationship with other biological molecules and its putative role in carcinogenesis.
dc.languageeng
dc.publisherLondon
dc.relationBiological Procedures Online
dc.rightsColombo et al.; licensee BioMed Central Ltd. - This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.rightsopenAccess
dc.subjectCircular dichroism
dc.subjectCloning
dc.subjectHSPC038
dc.subjectMolecular modeling
dc.subjectProtein expression
dc.subjectZNF706 protein
dc.titleExpression, purification and molecular analysis of the human ZNF706 protein
dc.typeArtículos de revistas


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