dc.creator | Zapata, Ronald Ordinola | |
dc.creator | Bramante, Clovis Monteiro | |
dc.creator | Minotti, Paloma Gagliardi | |
dc.creator | Cavenago, Bruno Cavalini | |
dc.creator | Garcia, Roberto Brandao | |
dc.creator | Bernardineli, Norberti | |
dc.creator | Jaramillo, David E. | |
dc.creator | Duarte, Marco Antonio Hungaro | |
dc.date.accessioned | 2013-12-10T18:37:28Z | |
dc.date.accessioned | 2018-07-04T16:29:26Z | |
dc.date.available | 2013-12-10T18:37:28Z | |
dc.date.available | 2018-07-04T16:29:26Z | |
dc.date.created | 2013-12-10T18:37:28Z | |
dc.date.issued | 2013-01 | |
dc.identifier | Journal of Endodontics, Chicago, v. 39, n. 1, p. 115-118, Jan. 2013 | |
dc.identifier | 0099-2399 | |
dc.identifier | http://www.producao.usp.br/handle/BDPI/43582 | |
dc.identifier | 10.1016/j.joen.2012.10.004 | |
dc.identifier | http://ac.els-cdn.com/S0099239912009375/1-s2.0-S0099239912009375-main.pdf?_tid=1938131c-61c0-11e3-bb59-00000aab0f27&acdnat=1386696523_5d2b59f3f73a8c4d2ea4ea2d88606daa | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1636302 | |
dc.description.abstract | Introduction: The purpose of this study was to evaluate the antimicrobial activity of calcium hydroxide, 2% chlorhexidine gel, and triantibiotic paste (ie, metronidazole, minocycline, and ciprofloxacin) by using an intraorally infected dentin biofilm model. Methods: Forty bovine dentin specimens were infected intraorally using a removable orthodontic device in order to induce the biofilm colonization of the dentin. Then, the samples were treated with the medications for 7 days. Saline solution was used as the control. Two evaluations were performed: immediately after the elimination of the medication and after incubation in brain-heart infusion medium for 24 hours. The Live/Dead technique (Invitrogen, Eugene, OR) and a confocal microscope were used to obtain the percentage of live cells. Nonparametric statistical tests were performed to show differences in the percentage of live cells among the groups (P < .05). Results: Calcium hydroxide and 2% chlorhexidine gel did not show statistical differences in the immediate evaluation. However, after application of the brain-heart infusion medium for 24 hours, 2% gel chlorhexidine showed a statistically lesser percentage of live cells in comparison with calcium hydroxide. The triantibiotic paste significantly showed a lower percentage of live cells in comparison with the 2% chlorhexidine gel and calcium hydroxide groups in the immediate and secondary (after 24 hours) evaluations. Conclusions: The triantibiotic paste was most effective at killing the bacteria in the biofilms on the intraorally infected dentin model in comparison with 2% chlorhexidine gel and calcium hydroxide | |
dc.language | eng | |
dc.publisher | Elsevier | |
dc.publisher | Chicago | |
dc.relation | Journal of Endodontics | |
dc.rights | Elsevier | |
dc.rights | restrictedAccess | |
dc.subject | Antimicrobials | |
dc.subject | bacteria | |
dc.subject | biofilms | |
dc.subject | dentin | |
dc.subject | root canal dressings | |
dc.title | Antimicrobial activity of triantibiotic paste, 2% chlorhexidine gel, and calcium hydroxide on an intraoral-infected dentin biofilm model | |
dc.type | Artículos de revistas | |