dc.creator | Victor, Jefferson R | |
dc.creator | Muniz, Bruno P | |
dc.creator | Fusaro, Ana E | |
dc.creator | de Brito, Cyro A | |
dc.creator | Taniguchi, Eliana F | |
dc.creator | Duarte, Alberto JS | |
dc.creator | Sato, Maria N | |
dc.date.accessioned | 2013-08-26T16:58:20Z | |
dc.date.accessioned | 2018-07-04T16:27:05Z | |
dc.date.available | 2013-08-26T16:58:20Z | |
dc.date.available | 2018-07-04T16:27:05Z | |
dc.date.created | 2013-08-26T16:58:20Z | |
dc.date.issued | 2010 | |
dc.identifier | BMC Immunology. 2010 Mar 11;11(1):11 | |
dc.identifier | 1471-2172 | |
dc.identifier | http://www.producao.usp.br/handle/BDPI/32795 | |
dc.identifier | 10.1186/1471-2172-11-11 | |
dc.identifier | http://www.biomedcentral.com/1471-2172/11/11 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1635947 | |
dc.description.abstract | Abstract
Background
Preconception allergen immunization prevents neonatal allergen sensitization in mice by a complex interaction between regulatory cells/factors and antibodies. The present study assessed the influence of maternal immunization with ovalbumin (OVA) on the immune response of 3 day-old and 3 week-old offspring immunized or non-immunized with OVA and evaluated the effect of IgG treatment during fetal development or neonatal period.
Results
Maternal immunization with OVA showed increased levels of FcγRIIb expression in splenic B cells of neonates, which were maintained for up to 3 weeks and not affected by additional postnatal OVA immunization. Maternal immunization also exerted a down-modulatory effect on both IL-4 and IFN-γ-secreting T cells and IL-4 and IL-12- secreting B cells. Furthermore, immunized neonates from immunized mothers showed a marked inhibition of antigen-specifc IgE Ab production and lowered Th2/Th1 cytokine levels, whereas displaying enhanced FcγRIIb expression on B cells. These offspring also showed reduced antigen-specific proliferative response and lowered B cell responsiveness. Moreover, in vitro evaluation revealed an impairment of B cell activation upon engagement of B cell antigen receptor by IgG from OVA-immunized mice. Finally, in vivo IgG transference during pregnancy or breastfeeding revealed that maternal Ab transference was able to increase regulatory cytokines, such as IL-10, in the prenatal stage; yet only the postnatal treatment prevented neonatal sensitization. None of the IgG treatments induced immunological changes in the offspring, as it was observed for those from OVA-immunized mothers.
Conclusion
Maternal immunization upregulates the inhibitory FcγRIIb expression on offspring B cells, avoiding skewed Th2 response and development of allergy. These findings contribute to the advancement of prophylactic strategies to prevent allergic diseases in early life. | |
dc.language | eng | |
dc.relation | BMC Immunology | |
dc.rights | Victor et al; licensee BioMed Central Ltd. - This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | |
dc.rights | openAccess | |
dc.title | Maternal immunization with ovalbumin prevents neonatal allergy development and up-regulates inhibitory receptor FcγRIIB expression on B cells | |
dc.type | Artículos de revistas | |