dc.creatorGalvao, Flavio H. F.
dc.creatorSoler, Wangles
dc.creatorPompeu, Eduardo
dc.creatorWaisberg, Daniel R.
dc.creatorMello, Evandro S. D.
dc.creatorCosta, Anderson C. L.
dc.creatorTeodoro, Walcy
dc.creatorVelosa, Ana P.
dc.creatorCapelozzi, Vera L.
dc.creatorAntonangelo, Leila
dc.creatorCatanozi, Sergio
dc.creatorMartins, Alessandro
dc.creatorMalbouisson, Luiz M. S.
dc.creatorCruz, Ruy J., Jr.
dc.creatorFigueira, Estela R.
dc.creatorFilho, Joel A. R.
dc.creatorChaib, Eleazar
dc.creatorD'Albuquerque, Luiz A. C.
dc.date.accessioned2013-10-21T13:36:26Z
dc.date.accessioned2018-07-04T16:26:07Z
dc.date.available2013-10-21T13:36:26Z
dc.date.available2018-07-04T16:26:07Z
dc.date.created2013-10-21T13:36:26Z
dc.date.issued2012
dc.identifierXENOTRANSPLANTATION, HOBOKEN, v. 19, n. 5, supl. 1, Part 2, pp. 298-304, SEP-OCT, 2012
dc.identifier0908-665X
dc.identifierhttp://www.producao.usp.br/handle/BDPI/35355
dc.identifier10.1111/xen.12002
dc.identifierhttp://dx.doi.org/10.1111/xen.12002
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1635799
dc.description.abstractGalvao FHF, Soler W, Pompeu E, Waisberg DR, Mello ES, Costa ACL, Teodoro W, Velosa AP, Capelozzi VL, Antonangelo L, Catanozi S, Martins A, Malbouisson LMS, Cruz RJ, Figueira ER, Filho JAR, Chaib E, D'Albuquerque LAC. Immunoglobulin G profile in hyperacute rejection after multivisceral xenotransplantation. Xenotransplantation 2012; 19: 298304. (c) 2012 John Wiley & Sons A/S. Abstract: Introduction: Xenotransplantation is a potential solution for the high mortality of patients on the waiting list for multivisceral transplantation; nevertheless, hyperacute rejection (HAR) hampers this practice and motivates innovative research. In this report, we describe a model of multivisceral xenotransplantation in which we observed immunoglobulin G (IgG) involvement in HAR. Methods: We recovered en bloc multivisceral grafts (distal esophagus, stomach, small intestine, colon, liver, pancreas, and kidneys) from rabbits (n = 20) and implanted them in the swine (n = 15) or rabbits (n = 5, control). Three hours after graft reperfusion, we collected samples from all graft organs for histological study and to assess IgG fixation by immunofluorescence. Histopathologic findings were graded according to previously described methods. Results: No histopathological features of rejection were seen in the rabbit allografts. In the swine-to-rabbit grafts, features of HAR were moderate in the liver and severe in esophagus, stomach, intestines, spleen, pancreas, and kidney. Xenograft vessels were the central target of HAR. The main lesions included edema, hemorrhage, thrombosis, myosites, fibrinoid degeneration, and necrosis. IgG deposition was intense on cell membranes, mainly in the vascular endothelium. Conclusions: Rabbit-to-swine multivisceral xenotransplants undergo moderate HAR in the liver and severe HAR in the other organs. Moderate HAR in the liver suggests a degree of resistance to the humoral immune response in this organ. Strong IgG fixation in cell membranes, including vascular endothelium, confirms HAR characterized by a primary humoral immune response. This model allows appraisal of HAR in multiple organs and investigation of the livers relative resistance to this immune response.
dc.languageeng
dc.publisherWILEY-BLACKWELL
dc.publisherHOBOKEN
dc.relationXENOTRANSPLANTATION
dc.rightsCopyright WILEY-BLACKWELL
dc.rightsclosedAccess
dc.subjectLIVER
dc.subjectMULTIVISCERAL
dc.subjectPIG
dc.subjectRABBIT
dc.subjectXENOTRANSPLANTATION
dc.titleImmunoglobulin G profile in hyperacute rejection after multivisceral xenotransplantation
dc.typeArtículos de revistas


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