dc.creator | Alonso Domingos, André Luis | |
dc.creator | Garcia, Sergio Britto | |
dc.creator | Bessa, Jose de, Jr. | |
dc.creator | Cassini, Marcelo Ferreira | |
dc.creator | Fernandes Molina, Carlos Augusto | |
dc.creator | Tucci Junior, Silvio | |
dc.date.accessioned | 2013-11-06T15:55:44Z | |
dc.date.accessioned | 2018-07-04T16:24:16Z | |
dc.date.available | 2013-11-06T15:55:44Z | |
dc.date.available | 2018-07-04T16:24:16Z | |
dc.date.created | 2013-11-06T15:55:44Z | |
dc.date.issued | 2012 | |
dc.identifier | INTERNATIONAL BRAZ J UROL, RIO DE JANEIRO, v. 38, n. 4, pp. 536-543, JUL-AUG, 2012 | |
dc.identifier | 1677-5538 | |
dc.identifier | http://www.producao.usp.br/handle/BDPI/42248 | |
dc.identifier | 10.1590/S1677-55382012000400014 | |
dc.identifier | http://dx.doi.org/10.1590/S1677-55382012000400014 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1635381 | |
dc.description.abstract | Objective: To investigate the VEGF expression and collagen deposition using a latex biomembrane as bladder replacement in rabbits. Materials and Methods: After partial cystectomy, a patch of a non-vulcanized latex biomembrane (2 x 2 cm) was sewn to the bladder of rabbits with 5/0 monofilament polydioxanone sulfate sutures in a watertight manner. Groups of 5 animals were killed at 15, 45 and 90 days after surgery and the bladder was removed. Sections of 5 mu m were cut and stained with picrosirius-red in order to estimate the amount of extracellular matrix in the graft. To confirm the presence of VEGF in tissues, protein expression was determined by immunohistochemistry. Results: No death, urinary leakage or graft extrusion occurred in any group. All bladders showed a spherical shape. A progressive reduction in the amount of collagen occurred in the graft area and was negatively and linearly correlated with time (p < 0.001). VEGF expression was higher in grafted areas when compared to controls at 15 and 45 days after surgery and decreased with time (p < 0.001). Conclusion: The latex biomembrane as a matrix for partial bladder replacement in rabbits promotes temporary collagen deposition and stimulates the angiogenic process. | |
dc.language | eng | |
dc.publisher | BRAZILIAN SOC UROL | |
dc.publisher | RIO DE JANEIRO | |
dc.relation | INTERNATIONAL BRAZ J UROL | |
dc.rights | Copyright BRAZILIAN SOC UROL | |
dc.rights | openAccess | |
dc.subject | LATEX | |
dc.subject | COLLAGEN | |
dc.subject | URINARY TRACT | |
dc.subject | TISSUE-ENGINEERING | |
dc.title | Expression of VEGF and collagen using a latex biomembrane as bladder replacement in rabbits | |
dc.type | Artículos de revistas | |