dc.creatorAlonso Domingos, André Luis
dc.creatorGarcia, Sergio Britto
dc.creatorBessa, Jose de, Jr.
dc.creatorCassini, Marcelo Ferreira
dc.creatorFernandes Molina, Carlos Augusto
dc.creatorTucci Junior, Silvio
dc.date.accessioned2013-11-06T15:55:44Z
dc.date.accessioned2018-07-04T16:24:16Z
dc.date.available2013-11-06T15:55:44Z
dc.date.available2018-07-04T16:24:16Z
dc.date.created2013-11-06T15:55:44Z
dc.date.issued2012
dc.identifierINTERNATIONAL BRAZ J UROL, RIO DE JANEIRO, v. 38, n. 4, pp. 536-543, JUL-AUG, 2012
dc.identifier1677-5538
dc.identifierhttp://www.producao.usp.br/handle/BDPI/42248
dc.identifier10.1590/S1677-55382012000400014 
dc.identifierhttp://dx.doi.org/10.1590/S1677-55382012000400014 
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1635381
dc.description.abstractObjective: To investigate the VEGF expression and collagen deposition using a latex biomembrane as bladder replacement in rabbits. Materials and Methods: After partial cystectomy, a patch of a non-vulcanized latex biomembrane (2 x 2 cm) was sewn to the bladder of rabbits with 5/0 monofilament polydioxanone sulfate sutures in a watertight manner. Groups of 5 animals were killed at 15, 45 and 90 days after surgery and the bladder was removed. Sections of 5 mu m were cut and stained with picrosirius-red in order to estimate the amount of extracellular matrix in the graft. To confirm the presence of VEGF in tissues, protein expression was determined by immunohistochemistry. Results: No death, urinary leakage or graft extrusion occurred in any group. All bladders showed a spherical shape. A progressive reduction in the amount of collagen occurred in the graft area and was negatively and linearly correlated with time (p < 0.001). VEGF expression was higher in grafted areas when compared to controls at 15 and 45 days after surgery and decreased with time (p < 0.001). Conclusion: The latex biomembrane as a matrix for partial bladder replacement in rabbits promotes temporary collagen deposition and stimulates the angiogenic process.
dc.languageeng
dc.publisherBRAZILIAN SOC UROL
dc.publisherRIO DE JANEIRO
dc.relationINTERNATIONAL BRAZ J UROL
dc.rightsCopyright BRAZILIAN SOC UROL
dc.rightsopenAccess
dc.subjectLATEX
dc.subjectCOLLAGEN
dc.subjectURINARY TRACT
dc.subjectTISSUE-ENGINEERING
dc.titleExpression of VEGF and collagen using a latex biomembrane as bladder replacement in rabbits
dc.typeArtículos de revistas


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