dc.creator | Sousa, João Paulo Barreto de | |
dc.creator | Brancalion, Ana Paula S. | |
dc.creator | Groppo Junior, Milton | |
dc.creator | Bastos, Jairo Kenupp | |
dc.date.accessioned | 2013-11-01T11:22:22Z | |
dc.date.accessioned | 2018-07-04T16:23:23Z | |
dc.date.available | 2013-11-01T11:22:22Z | |
dc.date.available | 2018-07-04T16:23:23Z | |
dc.date.created | 2013-11-01T11:22:22Z | |
dc.date.issued | 2012 | |
dc.identifier | NATURAL PRODUCT COMMUNICATIONS, WESTERVILLE, v. 7, n. 1, pp. 25-28, JAN, 2012 | |
dc.identifier | 1934-578X | |
dc.identifier | http://www.producao.usp.br/handle/BDPI/37403 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1635200 | |
dc.description.abstract | Hydroethanolic extracts of C. langsdorffii leaves have therapeutic potential. This work reports a validated chromatographic method for the quantification of polar compounds in the hydroethanolic extract of C. langsdorffii leaves. A reliable HPLC method was developed using two monolithic columns linked in series (100 x 4.6 mm - C-18), with nonlinear gradient elution, and UV detection set at 257 nm. A procedure for the extraction of flavonols was also developed, which involved the use of 70% aqueous ethanol and the addition of benzophenone as the internal standard. The developed method led to a good detection response as the values for linearity were between 10.3 and 1000 mu g/mL, and those for recovery between 84.2 and 111.1%. The detection limit ranged from 0.02 to 1.70 mu g/mL and the quantitation limit from 0.07 to 5.1 mu g/mL, with a maximum RSD of 5.24%. Five compounds, rutin, quercetin-3-O-alpha-L-rhamnopyranoside, kaempferol-3-O-alpha-L-rhamnopyranoside, quercetin and kaempferol, were quantified. This method could, therefore, be used for the quality control of hydroethanolic extracts of Copaifera leaves and their cosmetic and pharmaceutical products. | |
dc.language | eng | |
dc.publisher | NATURAL PRODUCTS INC | |
dc.publisher | WESTERVILLE | |
dc.relation | NATURAL PRODUCT COMMUNICATIONS | |
dc.rights | Copyright NATURAL PRODUCTS INC | |
dc.rights | closedAccess | |
dc.subject | COPAIFERA LANGSDORFFII | |
dc.subject | FLAVONOLS | |
dc.subject | HPLC | |
dc.subject | VALIDATED CHROMATOGRAPHIC METHOD | |
dc.title | A Validated Chromatographic Method for the Determination of Flavonoids in Copaifera langsdorffii by HPLC | |
dc.type | Artículos de revistas | |