Artículos de revistas
Phylogenetic and Similarity Analysis of HTLV-1 Isolates from HIV-Coinfected Patients from the South and Southeast Regions of Brazil
Fecha
2012Registro en:
AIDS RESEARCH AND HUMAN RETROVIRUSES, NEW ROCHELLE, v. 28, n. 1, pp. 110-114, JAN, 2012
0889-2229
10.1089/aid.2011.0117
Autor
Magri, Mariana Cavalheiro
Macedo Brigido, Luis Fernando de
Rodrigues, Rosangela
Morimoto, Helena Kaminami
Ferreira, Joao Leandro de Paula
Araujo, Adele Caterino de
Institución
Resumen
HTLV-1 is endemic in Brazil and HIV/ HTLV-1 coinfection has been detected, mostly in the northeast region. Cosmopolitan HTLV-1a is the main subtype that circulates in Brazil. This study characterized 17 HTLV-1 isolates from HIV coinfected patients of southern (n = 7) and southeastern (n = 10) Brazil. HTLV-1 provirus DNA was amplified by nested PCR (env and LTR) and sequenced. Env sequences (705 bp) from 15 isolates and LTR sequences (731 bp) from 17 isolates showed 99.5% and 98.8% similarity among sequences, respectively. Comparing these sequences with ATK (HTLV-1a) and Mel5 (HTLV-1c) prototypes, similarities of 99% and 97.4%, respectively, for env and LTR with ATK, and 91.6% and 90.3% with Mel5, were detected. Phylogenetic analysis showed that all sequences belonged to the transcontinental subgroup A of the Cosmopolitan subtype, clustering in two Latin American clusters.