dc.creatorRamos, Rodrigo Nalio
dc.creatorChin, Lilian Sally
dc.creatorSantos, Ana Paula S. A. dos
dc.creatorBergami-Santos, Patricia Cruz
dc.creatorLaginha, Fabio
dc.creatorBarbuto, Jose Alexandre M.
dc.date.accessioned2013-11-06T19:06:22Z
dc.date.accessioned2018-07-04T16:19:36Z
dc.date.available2013-11-06T19:06:22Z
dc.date.available2018-07-04T16:19:36Z
dc.date.created2013-11-06T19:06:22Z
dc.date.issued2012
dc.identifierJOURNAL OF LEUKOCYTE BIOLOGY, BETHESDA, v. 92, n. 3, pp. 673-682, SEP, 2012
dc.identifier0741-5400
dc.identifierhttp://www.producao.usp.br/handle/BDPI/42624
dc.identifier10.1189/jlb.0112048
dc.identifierhttp://dx.doi.org/10.1189/jlb.0112048
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1634418
dc.description.abstractDCs orchestrate immune responses contributing to the pattern of response developed. In cancer, DCs may play a dysfunctional role in the induction of CD4(+)CD25(+) Foxp3(+) Tregs, contributing to immune evasion. We show here that Mo-DCs from breast cancer patients show an altered phenotype and induce preferentially Tregs, a phenomenon that occurred regardless of DC maturation stimulus (sCD40L, cytokine cocktail, TNF-alpha, and LPS). The Mo-DCs of patients induced low proliferation of allogeneic CD3(+)CD25(neg)Foxp3(neg) cells, which after becoming CD25(+), suppressed mitogen-stimulated T cells. Contrastingly, Mo-DCs from healthy donors induced a stronger proliferative response, a low frequency of CD4(+)CD25(+)Foxp3(+) with no suppressive activity. Furthermore, healthy Mo-DCs induced higher levels of IFN-gamma, whereas the Mo-DCs of patients induced higher levels of bioactive TGF-beta 1 and IL-10 in cocultures with allogeneic T cells. Interestingly, TGF-beta 1 blocking with mAb in cocultures was not enough to completely revert the Mo-DCs of patients' bias toward Treg induction. Altogether, these findings should be considered in immunotherapeutic approaches for cancer based on Mo-DCs. J. Leukoc. Biol. 92: 673-682; 2012.
dc.languageeng
dc.publisherFEDERATION AMER SOC EXP BIOL
dc.publisherBETHESDA
dc.relationJOURNAL OF LEUKOCYTE BIOLOGY
dc.rightsCopyright FEDERATION AMER SOC EXP BIOL
dc.rightsopenAccess
dc.subjectCANCER
dc.subjectTUMOR IMMUNOLOGY
dc.subjectTOLERANCE
dc.titleMonocyte-derived dendritic cells from breast cancer patients are biased to induce CD4(+)CD25(+)Foxp3(+) regulatory T cells
dc.typeArtículos de revistas


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