dc.creator | Ramos, Rodrigo Nalio | |
dc.creator | Chin, Lilian Sally | |
dc.creator | Santos, Ana Paula S. A. dos | |
dc.creator | Bergami-Santos, Patricia Cruz | |
dc.creator | Laginha, Fabio | |
dc.creator | Barbuto, Jose Alexandre M. | |
dc.date.accessioned | 2013-11-06T19:06:22Z | |
dc.date.accessioned | 2018-07-04T16:19:36Z | |
dc.date.available | 2013-11-06T19:06:22Z | |
dc.date.available | 2018-07-04T16:19:36Z | |
dc.date.created | 2013-11-06T19:06:22Z | |
dc.date.issued | 2012 | |
dc.identifier | JOURNAL OF LEUKOCYTE BIOLOGY, BETHESDA, v. 92, n. 3, pp. 673-682, SEP, 2012 | |
dc.identifier | 0741-5400 | |
dc.identifier | http://www.producao.usp.br/handle/BDPI/42624 | |
dc.identifier | 10.1189/jlb.0112048 | |
dc.identifier | http://dx.doi.org/10.1189/jlb.0112048 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1634418 | |
dc.description.abstract | DCs orchestrate immune responses contributing to the pattern of response developed. In cancer, DCs may play a dysfunctional role in the induction of CD4(+)CD25(+) Foxp3(+) Tregs, contributing to immune evasion. We show here that Mo-DCs from breast cancer patients show an altered phenotype and induce preferentially Tregs, a phenomenon that occurred regardless of DC maturation stimulus (sCD40L, cytokine cocktail, TNF-alpha, and LPS). The Mo-DCs of patients induced low proliferation of allogeneic CD3(+)CD25(neg)Foxp3(neg) cells, which after becoming CD25(+), suppressed mitogen-stimulated T cells. Contrastingly, Mo-DCs from healthy donors induced a stronger proliferative response, a low frequency of CD4(+)CD25(+)Foxp3(+) with no suppressive activity. Furthermore, healthy Mo-DCs induced higher levels of IFN-gamma, whereas the Mo-DCs of patients induced higher levels of bioactive TGF-beta 1 and IL-10 in cocultures with allogeneic T cells. Interestingly, TGF-beta 1 blocking with mAb in cocultures was not enough to completely revert the Mo-DCs of patients' bias toward Treg induction. Altogether, these findings should be considered in immunotherapeutic approaches for cancer based on Mo-DCs. J. Leukoc. Biol. 92: 673-682; 2012. | |
dc.language | eng | |
dc.publisher | FEDERATION AMER SOC EXP BIOL | |
dc.publisher | BETHESDA | |
dc.relation | JOURNAL OF LEUKOCYTE BIOLOGY | |
dc.rights | Copyright FEDERATION AMER SOC EXP BIOL | |
dc.rights | openAccess | |
dc.subject | CANCER | |
dc.subject | TUMOR IMMUNOLOGY | |
dc.subject | TOLERANCE | |
dc.title | Monocyte-derived dendritic cells from breast cancer patients are biased to induce CD4(+)CD25(+)Foxp3(+) regulatory T cells | |
dc.type | Artículos de revistas | |