dc.creator | Ovando, Fernanda | |
dc.creator | Gimpel, Carla | |
dc.creator | Cardenas, Constanza | |
dc.creator | Silva, Jose Roberto Machado Cunha da | |
dc.creator | De Lorgeril, Julien | |
dc.creator | Gonzalez, Marcelo | |
dc.date.accessioned | 2013-11-05T14:51:54Z | |
dc.date.accessioned | 2018-07-04T16:18:20Z | |
dc.date.available | 2013-11-05T14:51:54Z | |
dc.date.available | 2018-07-04T16:18:20Z | |
dc.date.created | 2013-11-05T14:51:54Z | |
dc.date.issued | 2012 | |
dc.identifier | JOURNAL OF SHELLFISH RESEARCH, GROTON, v. 31, n. 3, supl. 4, Part 1, pp. 875-883, AUG, 2012 | |
dc.identifier | 0730-8000 | |
dc.identifier | http://www.producao.usp.br/handle/BDPI/41566 | |
dc.identifier | 10.2983/035.031.0336 | |
dc.identifier | http://dx.doi.org/10.2983/035.031.0336 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1634136 | |
dc.description.abstract | We have cloned and characterized for the first time an allograft inflammatory factor 1 (Sn-AIF-1) from the Antarctic sea urchin. We report the cloning of Sn-AIF-1 cDNA and the characterization of its expression in coelomocytes after a bacterial challenge. The cDNA Sn-AIF-1 has a size of 608 bp and encodes a polypeptide of 151 aa. The deduced amino acid sequence has a putative size of 17.430 Da, an isoelectric point of 4.92, and shows 2 elongation factor handlike motifs that normally bind calcium ions. BLAST analysis revealed close matches with other known AIF-1. The deduced amino acid sequence of Sn-AIF-1 showed high homology with AIF-1 in vertebrates such as fish, mice, and humans; and in the case of invertebrates, the major degree of identity (55%) was with a predicted sequence of the purple sea urchin AIF-1, and 52% corresponded to a sponge. Expression of Sn-AIF-1 mRNA was analyzed by qPCR. Sn-AIF-1 mRNA expression was measured from coelomocytes after a bacterial challenge using RT-PCR and revealed that the gene was upregulated after 24 h. Sn-AIF-1 could participate in the inflammatory response, particularly in the activation of coelomocytes and their survival. | |
dc.language | eng | |
dc.publisher | NATL SHELLFISHERIES ASSOC | |
dc.publisher | GROTON | |
dc.relation | JOURNAL OF SHELLFISH RESEARCH | |
dc.rights | Copyright NATL SHELLFISHERIES ASSOC | |
dc.rights | closedAccess | |
dc.subject | ANTARCTICA | |
dc.subject | SEA URCHIN | |
dc.subject | STERECHINUS NEUMAYERI | |
dc.subject | COELOMOCYTES | |
dc.subject | GENE EXPRESSION | |
dc.subject | AIF-1 | |
dc.title | CLONING AND EXPRESSION ANALYSIS OF ALLOGRAFT INFLAMMATORY FACTOR TYPE 1 IN COELOMOCYTES OF ANTARCTIC SEA URCHIN (STERECHINUS NEUMAYERI) | |
dc.type | Artículos de revistas | |