dc.creator | Podgaec, Sérgio | |
dc.creator | Rizzo, Luiz Vicente | |
dc.creator | Cordeiro Fernandes, Luiz Flavio | |
dc.creator | Baracat, Edmund Chada | |
dc.creator | Abrão, Mauricio Simões | |
dc.date.accessioned | 2013-11-06T18:33:50Z | |
dc.date.accessioned | 2018-07-04T16:17:54Z | |
dc.date.available | 2013-11-06T18:33:50Z | |
dc.date.available | 2018-07-04T16:17:54Z | |
dc.date.created | 2013-11-06T18:33:50Z | |
dc.date.issued | 2012 | |
dc.identifier | AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, HOBOKEN, v. 68, n. 4, pp. 301-308, OCT, 2012 | |
dc.identifier | 1046-7408 | |
dc.identifier | http://www.producao.usp.br/handle/BDPI/42459 | |
dc.identifier | 10.1111/j.1600-0897.2012.01173.x | |
dc.identifier | http://dx.doi.org/10.1111/j.1600-0897.2012.01173.x | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1634037 | |
dc.description.abstract | Problem To evaluate CD4+CD25highFoxp3+ cells and IL-6, IL-10, IL-17, and TGF-beta in the peritoneal fluid of women with endometriosis. Method of study A total of ninety-eight patients were studied: endometriosis (n = 70) and control (n = 28). First, peritoneal fluid lymphocytes were isolated, and CD4+CD25high cells were identified using flow cytometry. Then, RT-PCR was performed to verify Foxp3 expression in these cells. Also, IL-6, IL-10, IL-17, and TGF-beta concentration was determined. Results Of all the lymphocytes in the peritoneal fluid of women with endometriosis, 36.5% (median) were CD4+CD25high compared to only 1.15% (median) in the control group (P < 0.001). Foxp3 expression was similarly elevated in patients with the disease compared to those without (median, 50 versus 5; P < 0.001). IL-6 and TGF-beta were higher in endometriosis group (IL-6: 327.5 pg/mL versus 195.5 pg/mL; TGF-beta: 340 pg/mL versus 171.5 pg/mL; both P < 0.001). IL-10 and IL-17 showed no significant differences between the two groups. Conclusion The peritoneal fluid of patients with endometriosis had a higher percentage of CD4+CD25highFoxp3+ cells and also higher levels of IL-6 and TGF-beta compared to women without the disease. These findings suggest that CD4+CD25highFoxp3+ cells may play a role in the pathogenesis of endometriosis. | |
dc.language | eng | |
dc.publisher | WILEY-BLACKWELL | |
dc.publisher | HOBOKEN | |
dc.relation | AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY | |
dc.rights | Copyright WILEY-BLACKWELL | |
dc.rights | closedAccess | |
dc.subject | CYTOKINES | |
dc.subject | ENDOMETRIOSIS | |
dc.subject | TREG CELLS | |
dc.title | CD4+CD25highFoxp3+Cells Increased in the Peritoneal Fluid of Patients with Endometriosis | |
dc.type | Artículos de revistas | |