dc.creator | Masui, Douglas Chodi | |
dc.creator | Zimbardi, Ana Lucia Ribeiro Latorre | |
dc.creator | Souza, Flavio Henrique Moreira de | |
dc.creator | Guimarães, Luis Henrique Souza | |
dc.creator | Furriel, Rosa dos Prazeres Melo | |
dc.creator | Jorge, Joao Atilio | |
dc.date.accessioned | 2013-11-04T14:33:42Z | |
dc.date.accessioned | 2018-07-04T16:15:51Z | |
dc.date.available | 2013-11-04T14:33:42Z | |
dc.date.available | 2018-07-04T16:15:51Z | |
dc.date.created | 2013-11-04T14:33:42Z | |
dc.date.issued | 2012 | |
dc.identifier | WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, NEW YORK, v. 28, n. 8, supl. 1, Part 1, pp. 2689-2701, AUG, 2012 | |
dc.identifier | 0959-3993 | |
dc.identifier | http://www.producao.usp.br/handle/BDPI/40869 | |
dc.identifier | 10.1007/s11274-012-1079-1 | |
dc.identifier | http://dx.doi.org/10.1007/s11274-012-1079-1 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1633583 | |
dc.description.abstract | Humicola brevis var. thermoidea cultivated under solid state fermentation in wheat bran and water (1:2 w/v) was a good producer of beta-glucosidase and xylanase. After optimization using response surface methodology the level of xylanase reached 5,791.2 +/- A 411.2 U g(-1), while beta-glucosidase production was increased about 2.6-fold, reaching 20.7 +/- A 1.5 U g(-1). Cellulase levels were negligible. Biochemical characterization of H. brevis beta-glucosidase and xylanase activities showed that they were stable in a wide pH range. Optimum pH for beta-glucosidase and xylanase activities were 5.0 and 5.5, respectively, but the xylanase showed 80 % of maximal activity when assayed at pH 8.0. Both enzymes presented high thermal stability. The beta-glucosidase maintained about 95 % of its activity after 26 h in water at 55 A degrees C, with half-lives of 15.7 h at 60 A degrees C and 5.1 h at 65 A degrees C. The presence of xylose during heat treatment at 65 A degrees C protected beta-glucosidase against thermal inactivation. Xylanase maintained about 80 % of its activity after 200 h in water at 60 A degrees C. Xylose stimulated beta-glucosidase activity up to 1.7-fold, at 200 mmol L-1. The notable features of both xylanase and beta-glucosidase suggest that H. brevis crude culture extract may be useful to compose efficient enzymatic cocktails for lignocellulosic materials treatment or paper pulp biobleaching. | |
dc.language | eng | |
dc.publisher | SPRINGER | |
dc.publisher | NEW YORK | |
dc.relation | WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | |
dc.rights | Copyright SPRINGER | |
dc.rights | restrictedAccess | |
dc.subject | BETA-GLUCOSIDASE | |
dc.subject | THERMOSTABLE XYLANASE | |
dc.subject | XYLOSE STIMULATED BETA-GLUCOSIDASE | |
dc.subject | HUMICOLA BREVIS | |
dc.subject | THERMOPHILIC FUNGI | |
dc.title | Production of a xylose-stimulated beta-glucosidase and a cellulase-free thermostable xylanase by the thermophilic fungus Humicola brevis var. thermoidea under solid state fermentation | |
dc.type | Artículos de revistas | |