dc.creator | Barbosa Chaves, Karen Cristina | |
dc.creator | Turaca, Lauro Thiago | |
dc.creator | Pesquero, Joao Bosco | |
dc.creator | Mennecier, Gregory | |
dc.creator | Zaidan Dagli, Maria Lucia | |
dc.creator | Chammas, Roger | |
dc.creator | Schor, Nestor | |
dc.creator | Bellini, Maria Helena | |
dc.date.accessioned | 2013-10-29T15:04:26Z | |
dc.date.accessioned | 2018-07-04T16:15:30Z | |
dc.date.available | 2013-10-29T15:04:26Z | |
dc.date.available | 2018-07-04T16:15:30Z | |
dc.date.created | 2013-10-29T15:04:26Z | |
dc.date.issued | 2012 | |
dc.identifier | BIOMEDICINE & PHARMACOTHERAPY, PARIS, v. 66, n. 6, supl. 1, Part 3, pp. 464-468, SEP, 2012 | |
dc.identifier | 0753-3322 | |
dc.identifier | http://www.producao.usp.br/handle/BDPI/36499 | |
dc.identifier | 10.1016/j.biopha.2012.04.003 | |
dc.identifier | http://dx.doi.org/10.1016/j.biopha.2012.04.003 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1633527 | |
dc.description.abstract | Tumor cells induce the disruption of homeostasis between cellular and extracellular compartments to favor tumor progression. The expression of fibronectin (FN), a matrix glycoprotein, is increased in several carcinoma cell types, including renal cell carcinoma (RCC). RCC are highly vascularized tumors and are often amenable to antiangiogenic therapy. Endostatin (ES) is a fragment of collagen XVIII that possesses antiangiogenic activity. In this study, we examined the modulation of FN gene expression by ES gene therapy in a murine metastatic renal cell carcinoma (mRCC) model. Balb/C mice bearing Renca cells were treated with NIH/3T3-LXSN cells or NIH/3T3-LendSN cells. At the end of the experiment, the ES serum levels were measured, and the FN gene expression was assessed using real-time PCR. The tissue FN was evaluated by western blotting and by immunofluorescence analysis. The ES serum levels in treated mice were higher than those in the control group (P < 0.05). ES treatment led to significant decreases at the FN mRNA (P < 0.001) and protein levels (P < 0.01). Here, we demonstrate the ES antitumor effect that is mediated by down-regulation of FN expression in mRCC. (C) 2012 Elsevier Masson SAS. All rights reserved. | |
dc.language | eng | |
dc.publisher | ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER | |
dc.publisher | PARIS | |
dc.relation | BIOMEDICINE & PHARMACOTHERAPY | |
dc.rights | Copyright ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER | |
dc.rights | closedAccess | |
dc.subject | RCC | |
dc.subject | ENDOSTATIN | |
dc.subject | ORTHOTOPIC METASTATIC MODEL | |
dc.subject | FIBRONECTIN | |
dc.title | Fibronectin expression is decreased in metastatic renal cell carcinoma following endostatin gene therapy | |
dc.type | Artículos de revistas | |