SK-HEP cells and lentiviral vector for production of human recombinant factor VIII

dc.creatorRosa, Nathalia Gonsales da
dc.creatorSwiech, Kamilla
dc.creatorPicanco-Castro, Virginia
dc.creatorRusso-Carbolante, Elisa Maria de Sousa
dc.creatorSoares Neto, Mario Abreu
dc.creatorCastilho-Fernandes, Andrielle de
dc.creatorFaca, Vitor Marcel
dc.creatorFontes, Aparecida Maria
dc.creatorCovas, Dimas Tadeu
dc.date.accessioned2013-09-24T15:08:58Z
dc.date.accessioned2018-07-04T16:15:10Z
dc.date.available2013-09-24T15:08:58Z
dc.date.available2018-07-04T16:15:10Z
dc.date.created2013-09-24T15:08:58Z
dc.date.issued2012
dc.identifierBIOTECHNOLOGY LETTERS, DORDRECHT, v. 34, n. 8, pp. 1435-1443, AUG, 2012
dc.identifier0141-5492
dc.identifierhttp://www.producao.usp.br/handle/BDPI/33648
dc.identifier10.1007/s10529-012-0925-4
dc.identifierhttp://dx.doi.org/10.1007/s10529-012-0925-4
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1633451
dc.description.abstractHemophilia A is caused by a deficiency in coagulation factor VIII. Recombinant factor VIII can be used as an alternative although it is unavailable for most patients. Here, we describe the production of a human recombinant B-domain-deleted FVIII (rBDDFVIII) by the human cell line SK-HEP-1, modified by a lentiviral vector rBDDFVIII was produced by recombinant SK-HEP cells (rSK-HEP) at 1.5-2.1 IU/10(6) in 24 h. The recombinant factor had increased in vitro stability when compared to commercial pdFVIII. The functionality of rBDDFVIII was shown by its biological activity and by tail-clip challenge in hemophilia A mice. The rSK-HEP cells grew in a scalable system and produced active rBDDFVIII, indicating that this platform production can be optimized to meet the commercial production scale needs.
dc.languageeng
dc.publisherSPRINGER
dc.publisherDORDRECHT
dc.relationBIOTECHNOLOGY LETTERS
dc.rightsCopyright SPRINGER
dc.rightsclosedAccess
dc.subjectHUMAN CELL LINE
dc.subjectHUMAN FACTOR VIII
dc.subjectLENTIVIRAL VECTOR
dc.subjectMICROCARRIER CULTURE
dc.subjectSK-HEP CELLS
dc.subjectRECOMBINANT FACTOR VIII
dc.titleSK-HEP cells and lentiviral vector for production of human recombinant factor VIII
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución