dc.creator | Ferreira, Adilson Kleber | |
dc.creator | Meneguelo, Renato | |
dc.creator | Navarro Marques, Fabio Luiz | |
dc.creator | Radin, Adriano | |
dc.creator | Filho, Otaviano Mendonca R. | |
dc.creator | Neto, Salvador Claro | |
dc.creator | Chierice, Gilberto Orivaldo | |
dc.creator | Maria, Durvanei Augusto | |
dc.date.accessioned | 2013-11-05T14:24:18Z | |
dc.date.accessioned | 2018-07-04T16:14:14Z | |
dc.date.available | 2013-11-05T14:24:18Z | |
dc.date.available | 2018-07-04T16:14:14Z | |
dc.date.created | 2013-11-05T14:24:18Z | |
dc.date.issued | 2012 | |
dc.identifier | BIOMEDICINE & PHARMACOTHERAPY, PARIS, v. 66, n. 7, supl. 1, Part 3, pp. 541-548, OCT, 2012 | |
dc.identifier | 0753-3322 | |
dc.identifier | http://www.producao.usp.br/handle/BDPI/41511 | |
dc.identifier | 10.1016/j.biopha.2012.04.008 | |
dc.identifier | http://dx.doi.org/10.1016/j.biopha.2012.04.008 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1633248 | |
dc.description.abstract | Phosphoethanolamine (Pho-s) is a compound involved in phospholipid turnover, acting as a substrate for many phospholipids of the cell membranes, especially phosphatidylcholine. We recently reported that synthetic Pho-s has potent effects on a wide variety of tumor cells. To determine if Pho-s has a potential antitumor activity, in this study we evaluated the activity of Pho-s against the B16-F10 melanoma both in vitro and in mice bearing a dorsal tumor. The treatment of B16F10 cells with Pho-s resulted in a dose-dependent inhibition of cell proliferation. At low concentrations, this activity appears to be involved in the arrest of the cell cycle at G2/M, while at high concentrations Pho-s induces apoptosis. In accordance with these results, the loss of mitochondrial potential and increased caspase-3 activity suggest that Phos has dual antitumor effects; i.e. it induces apoptosis at high concentrations and modulates the cell cycle at lower concentrations. In vivo, we evaluated the effect of Pho-s in mice bearing B16-F10 melanoma. The results show that Pho-s reduces the tumoral volume increasing survival rate. Furthermore, the tumor doubling time and tumor delays were substantially reduced when compared with untreated mice. Histological analyses reveal that Pho-s induces changes in cell morphology, typical characteristics of apoptosis, in addition the large areas of necrosis correlating with a reduction of tumor size. The results presented here support the hypothesis that Pho-s has antitumor effects by the induction of apoptosis as well as the inhibition of cell proliferation by arrest at G2/M. Thus, Pho-s can be regarded as a promising agent for the treatment of melanoma. Published by Elsevier Masson SAS. | |
dc.language | eng | |
dc.publisher | ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER | |
dc.publisher | PARIS | |
dc.relation | BIOMEDICINE & PHARMACOTHERAPY | |
dc.rights | Copyright ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER | |
dc.rights | closedAccess | |
dc.subject | SYNTHETIC PHOSPHOETHANOLAMINE | |
dc.subject | APOPTOSIS | |
dc.subject | ANTITUMOR | |
dc.subject | CELL PROLIFERATION | |
dc.subject | CELL CYCLE ARREST | |
dc.title | Synthetic phosphoethanolamine a precursor of membrane phospholipids reduce tumor growth in mice bearing melanoma B16-F10 and in vitro induce apoptosis and arrest in G2/M phase | |
dc.type | Artículos de revistas | |