Brasil | Artículos de revistas
dc.creatorde Azevedo, M. S. P.
dc.creatorRocha, C. S.
dc.creatorElecto, N.
dc.creatorPontes, D. S.
dc.creatorMolfetta, J. B.
dc.creatorGoncalves, E. D. C.
dc.creatorAzevedo, V.
dc.creatorSilva, C. L.
dc.creatorMiyoshi, A.
dc.date.accessioned2013-11-01T15:50:01Z
dc.date.accessioned2018-07-04T16:09:05Z
dc.date.available2013-11-01T15:50:01Z
dc.date.available2018-07-04T16:09:05Z
dc.date.created2013-11-01T15:50:01Z
dc.date.issued2013-08-02
dc.identifierGENETICS AND MOLECULAR RESEARCH, RIBEIRAO PRETO, v. 11, n. 2, supl. 1, Part 3, pp. 1146-1157, AUG 7, 2012
dc.identifier1676-5680
dc.identifierhttp://www.producao.usp.br/handle/BDPI/37645
dc.identifier10.4238/2012.April.27.14
dc.identifierhttp://dx.doi.org/10.4238/2012.April.27.14
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1632092
dc.description.abstractLactic acid bacteria (LAB) are an attractive and safe alternative for the expression of heterologous proteins, as they are nonpathogenic and endotoxin-free organisms. Lactococcus lactis, the LAB model organism, has been extensively employed in the biotechnology field for large-scale production of heterologous proteins, and its use as a "cell factory" has been widely studied. We have been particularly interested in the use of L. lactis for production of heat shock proteins (HSPs), which reportedly play important roles in the initiation of innate and adaptive immune responses. However, this activity has been questioned, as LPS contamination appears to be responsible for most, if not all, immunostimulatory activity of HSPs. In order to study the effect of pure HSPs on the immune system, we constructed recombinant L. lactis strains able to produce and properly address the Mycobacterium leprae 65-kDa HSP (Hsp65) to the cytoplasm or to the extracellular medium, using a xylose-induced expression system. Approximately 7 mg/L recombinant Hsp65 was secreted. Degradation products related to lactococcal HtrA activity were not observed, and the Limulus amebocyte lysate assay demonstrated that the amount of LPS in the recombinant Hsp65 preparations was 10-100 times lower than the permitted levels established by the U. S. Food and Drug Administration. These new L. lactis strains will allow investigation of the effects of M. leprae Hsp65 without the interference of LPS; consequently, they have potential for a variety of biotechnological, medical and therapeutic applications.
dc.languageeng
dc.publisherFUNPEC-EDITORA
dc.publisherRIBEIRAO PRETO
dc.relationGENETICS AND MOLECULAR RESEARCH
dc.rightsCopyright FUNPEC-EDITORA
dc.rightsopenAccess
dc.subjectHEAT SHOCK PROTEINS
dc.subjectLPS-FREE HSP65
dc.subjectLACTOCOCCUS LACTIS
dc.subjectMYCOBACTERIUM LEPRAE
dc.titleCytoplasmic and extracellular expression of pharmaceutical-grade mycobacterial 65-kDa heat shock protein in Lactococcus lactis
dc.typeArtículos de revistas


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