Artículos de revistas
Transesterification of Palm Oil Catalyzed by Pseudomonas fluorescens Lipase in a Packed-Bed Reactor
Fecha
2013-08-02Registro en:
ENERGY & FUELS, WASHINGTON, v. 26, n. 9, supl. 4, Part 1-2, pp. 5977-5982, SEP, 2012
0887-0624
10.1021/ef300931y
Autor
Dors, Gisanara
Freitas, Larissa
Mendes, Adriano A.
Furigo, Agenor, Jr.
de Castro, Heizir F.
Institución
Resumen
Transesterification of palm oil with ethanol catalyzed by Pseudomonas fluorescens lipase immobilized on epoxy-polysiloxane-polyvinyl alcohol composite (epoxy-SiO2-PVA) was performed in a continuous packed-bed reactor (PBR). Two strategies were used for improving the miscibility of the substrates: the addition of the organic solvent tert-butanol and the surfactant Triton X-100. Results were compared to those obtained in a solventless reactor, which displayed a biphasic system that passed through the reactor. Using this system, the ethyl ester yield of 61.6 +/- 1.2% was obtained at steady state. Both Triton X-100 and tert-butanol systems were found to be suitable to promote the miscibility of the starting materials; however, the use of Triton X-100 reduced the yield to levels lower than 20%, because of the enzyme desorption from the support surface, as confirmed by scanning electron microscopy analysis. The best performance was found for the reactor running in the presence of tert-butanol which resulted in a stable operating system and an average yield of 87.6 +/- 2.5%. This strategy also gave high biocatalyst operational stability, revealing a half-life of 48 days and an inactivation constant of 0.6 X 10(-3) h(-1).