Artículos de revistas
MIF induces osteoclast differentiation and contributes to progression of periodontal disease in mice
Fecha
2013-08-02Registro en:
MICROBES AND INFECTION, AMSTERDAM, v. 14, n. 2, supl. 1, Part 4, pp. 198-206, FEB, 2012
1286-4579
10.1016/j.micinf.2011.09.005
Autor
Moreira Madeira, Mila Fernandes
Queiroz-Junior, Celso Martins
Costa, Graciela Mitre
Santos, Patricia Campi
Silveira, Elcia Maria
Garlet, Gustavo Pompermaier
Cisalpino, Patricia Silva
Teixeira, Mauro Martins
Silva, Tarcilia Aparecida
Souza, Daniele da Gloria
Institución
Resumen
Periodontal disease (PD) is a chronic inflammatory and alveolar bone destructive disease triggered by microorganisms from the oral biofilm. Oral inoculation of mice with the periodontopathogen Aggregatibacter actinomycetemcomitans (Aa) induces marked alveolar bone loss and local production of inflammatory mediators, including Macrophage Migration Inhibitory Factor (MW). The role of MW for alveolar bone resorption during PD is not known. In the present study, experimental PD was induced in BALB/c wild-type mice (WT) and MW knockout mice (MIF-/-) through oral inoculation of Aa. Despite enhanced number of bacteria, MIF-/- mice had reduced infiltration of TRAP-positive cells and reduced alveolar bone loss. This was associated with decreased neutrophil accumulation and increased levels of IL-10 in periodontal tissues. TNF-alpha production was similar in both groups. In vitro, LPS from Aa enhanced osteoclastic activity in a MIF-dependent manner. In conclusion, MIF has role in controlling bacterial growth in the context of PD but contributes more significantly to the progression of bone loss during PD by directly affecting differentiation and activity of osteoclasts. (C) 2011 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.