dc.creator | Slavov, Svetoslav Nanev | |
dc.creator | Kashima, Simone | |
dc.creator | Silva-Pinto, Ana Cristina | |
dc.creator | Covas, Dimas Tadeu | |
dc.date.accessioned | 2013-10-14T12:55:31Z | |
dc.date.accessioned | 2018-07-04T15:58:08Z | |
dc.date.available | 2013-10-14T12:55:31Z | |
dc.date.available | 2018-07-04T15:58:08Z | |
dc.date.created | 2013-10-14T12:55:31Z | |
dc.date.issued | 2012 | |
dc.identifier | CANADIAN JOURNAL OF MICROBIOLOGY, OTTAWA, v. 58, n. 2, supl. 4, Part 1, pp. 200-205, FEB, 2012 | |
dc.identifier | 0008-4166 | |
dc.identifier | http://www.producao.usp.br/handle/BDPI/34507 | |
dc.identifier | 10.1139/W11-119 | |
dc.identifier | http://dx.doi.org/10.1139/W11-119 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1629845 | |
dc.description.abstract | Human parvovirus B19 (B19V) infection can be a life-threatening condition among patients with hereditary (chronic) hemolytic anemias. Our objective was to characterize the infection molecularly among patients with sickle cell disease and thalassemia. Forty-seven patients (37 with sickle cell disease, and 10 with beta-thalassemia major) as well as 47 healthy blood donors were examined for B19V infection by anti-B19V IgG enzyme immunoassay, quantitative PCR, which detects all B19V genotypes, and DNA sequencing. B19V viremia was documented in nine patients (19.1%) as two displayed acute infection and the rest had a low titre viremia (mean 3.4 x 10(4) copies/mL). All donors were negative for B19V DNA. Anti-B19V IgG was detected in 55.3% of the patients and 57.4% among the donors. Based on partial NS1 fragments, all patient isolates were classified as genotype 1 and subgenotype 1A. The evolutionary events of the examined partial NS1 gene sequence were associated with a lack of positive selection. The quantification of all B19V genotypes by a single hydrolytic probe is a technically useful method, but it is difficult to establish relationships between B19V sequence characteristics and infection outcome. | |
dc.language | eng | |
dc.publisher | CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS | |
dc.publisher | OTTAWA | |
dc.relation | CANADIAN JOURNAL OF MICROBIOLOGY | |
dc.rights | Copyright CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS | |
dc.rights | restrictedAccess | |
dc.subject | HUMAN PARVOVIRUS B19 | |
dc.subject | SICKLE CELL DISEASE | |
dc.subject | THALASSEMIA | |
dc.subject | REAL-TIME PCR | |
dc.title | Genotyping of Human parvovirus B19 among Brazilian patients with hemoglobinopathies | |
dc.type | Artículos de revistas | |