dc.creatorSesso, A.
dc.creatorBelizario, J. E.
dc.creatorMarques, M. M.
dc.creatorHiguchi, M. L.
dc.creatorSchumacher, R. I.
dc.creatorColquhoun, A.
dc.creatorIto, E.
dc.creatorKawakami, J.
dc.date.accessioned2013-08-15T12:11:48Z
dc.date.accessioned2018-07-04T15:54:59Z
dc.date.available2013-08-15T12:11:48Z
dc.date.available2018-07-04T15:54:59Z
dc.date.created2013-08-15T12:11:48Z
dc.date.issued2012-10
dc.identifierANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY, HOBOKEN, v. 295, n. 10, supl. 1, Part 3, pp. 1647-1659, OCT, 2012
dc.identifier1932-8486
dc.identifierhttp://www.producao.usp.br/handle/BDPI/32555
dc.identifier10.1002/ar.22553
dc.identifierhttp://dx.doi.org/10.1002/ar.22553
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1629154
dc.description.abstractOuter mitochondrial membrane (OMM) rupture was first noted in isolated mitochondria in which the inner mitochondrial membrane (IMM) had lost its selective permeability. This phenomenon referred to as mitochondrial permeability transition (MPT) refers to a permeabilized inner membrane that originates a large swelling in the mitochondrial matrix, which distends the outer membrane until it ruptures. Here, we have expanded previous electron microscopic observations that in apoptotic cells, OMM rupture is not caused by a membrane stretching promoted by a markedly swollen matrix. It is shown that the widths of the ruptured regions of the OMM vary from 6 to 250 nm. Independent of the perforation size, herniation of the mitochondrial matrix appeared to have resulted in pushing the IMM through the perforation. A large, long focal herniation of the mitochondrial matrix, covered with the IMM, was associated with a rupture of the OMM that was as small as 6 nm. Contextually, the collapse of the selective permeability of the IMM may precede or follow the release of the mitochondrial proteins of the intermembrane space into the cytoplasm. When the MPT is a late event, exit of the intermembrane space proteins to the cytoplasm is unimpeded and occurs through channels that transverse the outer membrane, because so far, the inner membrane is impermeable. No channel within the outer membrane can expose to the cytoplasm a permeable inner membrane, because it would serve as a conduit for local herniation of the mitochondrial matrix. Anat Rec, 2012. (c) 2012 Wiley Periodicals, Inc.
dc.languageeng
dc.publisherWILEY-BLACKWELL
dc.publisherHOBOKEN
dc.relationANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY
dc.rightsCopyright WILEY-BLACKWELL
dc.rightsopenAccess
dc.subjectELECTRON MICROSCOPY
dc.subjectMITOCHONDRIA
dc.subjectAPOPTOSIS
dc.subjectRUPTURE OF THE OUTER MITOCHONDRIAL MEMBRANE
dc.subjectFOCAL MATRIX HERNIATION IN MITOCHONDRIA
dc.subjectINNER MITOCHONDRIAL MEMBRANE
dc.subjectMITOCHONDRIAL OUTER MEMBRANE PERMEABILIZATION
dc.subjectMITOCHONDRIAL PROTEINS OF THE INTERMEMBRANE SPACE
dc.subjectMITOCHON-DRIAL PERMEABILITY TRANSITION
dc.subjectOUTER MITOCHON-DRIAL MEMBRANE
dc.titleMitochondrial Swelling and Incipient Outer Membrane Rupture in Preapoptotic and Apoptotic Cells
dc.typeArtículos de revistas


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