dc.creator | SIMIONATO, Ana V. Colnaghi | |
dc.creator | MORAES, Edgar Perin | |
dc.creator | CARRILHO, Emanuel | |
dc.creator | TAVARES, Marina Franco Maggi | |
dc.creator | KENNDLER, Ernst | |
dc.date.accessioned | 2012-10-20T05:33:41Z | |
dc.date.accessioned | 2018-07-04T15:51:37Z | |
dc.date.available | 2012-10-20T05:33:41Z | |
dc.date.available | 2018-07-04T15:51:37Z | |
dc.date.created | 2012-10-20T05:33:41Z | |
dc.date.issued | 2008 | |
dc.identifier | ELECTROPHORESIS, v.29, n.10, p.2051-2058, 2008 | |
dc.identifier | 0173-0835 | |
dc.identifier | http://producao.usp.br/handle/BDPI/31756 | |
dc.identifier | 10.1002/elps.200700629 | |
dc.identifier | http://dx.doi.org/10.1002/elps.200700629 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1628394 | |
dc.description.abstract | In this work, a CE equipment, online hyphenated to an IT MS analyzer by a linear sheath liquid interface promoting ESI, was used to develop a method for quantitative determination of amino acids. Under appropriate conditions (BGE composition, 0.8% HCOOH, 20% CH(3)OH; sheath liquid composition, 0.8% HCOOH, 60% methanol; V(ESI), +4.50 W), analytical curves of all amino acids from 3 to 80 mg/L were recorded presenting acceptable linearity (r > 0.99). LODs in the range of 16-172 mu mol/L were obtained. BSA, a model protein, was submitted to different hydrolysis procedures (classical acid and basic, and catalyzed by the H(+) form of a cation exchanger resin) and its amino acid profiles determined. In general, the resin-mediated hydrolysis yields were overall similar or better than those obtained by classical acid or basic hydrolysis. The resulting experimental-to-theoretical BSA concentration ratios served as correction factors for the quantitation of amino acids in Brazil nut resin generated hydrolysates. | |
dc.language | eng | |
dc.publisher | WILEY-BLACKWELL | |
dc.relation | Electrophoresis | |
dc.rights | Copyright WILEY-BLACKWELL | |
dc.rights | restrictedAccess | |
dc.subject | amino acids | |
dc.subject | cation exchanger resin | |
dc.subject | capillary electrophoresis-electrospray ionization-mass spectrometry | |
dc.subject | hydrolysis methods | |
dc.title | Determination of amino acids by capillary electrophoresis-electrospray ionization-mass spectrometry: An evaluation of different protein hydrolysis procedures | |
dc.type | Artículos de revistas | |