Artículos de revistas
Mitochondrial bioenergetics and redox state are unaltered in Trypanosoma cruzi isolates with compromised mitochondrial complex I subunit genes
Fecha
2009Registro en:
JOURNAL OF BIOENERGETICS AND BIOMEMBRANES, v.41, n.3, p.299-308, 2009
0145-479X
10.1007/s10863-009-9228-4
Autor
CARRANZA, Julio Cesar
KOWALTOWSKI, Alicia J.
MENDONCA, Marco Aurelio G.
OLIVEIRA, Thays C. de
GADELHA, Fernanda R.
ZINGALES, Bianca
Institución
Resumen
In trypanosomatids the involvement of mitochondrial complex I in NADH oxidation has long been debated. Here, we took advantage of natural Trypanosoma cruzi mutants which present conspicuous deletions in ND4, ND5 and ND7 genes coding for complex I subunits to further investigate its functionality. Mitochondrial bioenergetics of wild type and complex I mutants showed no significant differences in oxygen consumption or respiratory control ratios in the presence of NADH-linked substrates or FADH(2)-generating succinate. No correlation could be established between mitochondrial membrane potentials and ND deletions. Since release of reactive oxygen species occurs at complex I, we measured mitochondrial H(2)O(2) formation induced by different substrates. Significant differences not associated to ND deletions were observed among the parasite isolates, demonstrating that these mutations are not important for the control of oxidant production. Our data support the notion that complex I has a limited function in T. cruzi.
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