dc.creatorSCULACCIO, Susana Andrea
dc.creatorRODRIGUES, E. M.
dc.creatorCORDEIRO, A. T.
dc.creatorMAGALHAES, A.
dc.creatorBRAGA, A. L.
dc.creatorALBERTO, E. E.
dc.creatorTHIEMANN, Otavio Henrique
dc.date.accessioned2012-10-20T04:25:29Z
dc.date.accessioned2018-07-04T15:44:57Z
dc.date.available2012-10-20T04:25:29Z
dc.date.available2018-07-04T15:44:57Z
dc.date.created2012-10-20T04:25:29Z
dc.date.issued2008
dc.identifierMOLECULAR AND BIOCHEMICAL PARASITOLOGY, v.162, n.2, p.165-171, 2008
dc.identifier0166-6851
dc.identifierhttp://producao.usp.br/handle/BDPI/30196
dc.identifier10.1016/j.molbiopara.2008.08.009
dc.identifierhttp://dx.doi.org/10.1016/j.molbiopara.2008.08.009
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1626836
dc.description.abstractSelenophosphate synthetase (EC 2.7.9.3), the product of the selD gene, produces the biologically selenium donor compound, monoselenophosphate, from ATP and selenide, for the synthesis of cysteine. The kinetoplastid Leishmania major and Trypanosoma brucei selD genes were cloned and the protein overexpressed and purified to apparent homogeneity. The selD gene in L. major and T brucei respectively 1197 and 1179 bp long encoding proteins of 399 and 393 amino acids with molecular of 42.7 and 43 kDa. The molecular mass of 100 kDa for both (L. major and T brucei) SEWS is consistent dimeric proteins. The kinetoplastid selD complement Escherichia call (WL400) selD deletion it is a functional enzyme and the specific activity of these enzymes was determined. A conserved residue was identified both by multiple sequence alignment as well as by functional and activity assay of the mutant (Cys to Ala) forms of the SELD identifying this residue as essential for catalytic function. (C) 2008 Elsevier B.V. All rights reserved.
dc.languageeng
dc.publisherELSEVIER SCIENCE BV
dc.relationMolecular and Biochemical Parasitology
dc.rightsCopyright ELSEVIER SCIENCE BV
dc.rightsrestrictedAccess
dc.subjectSelenophosphate synthetase
dc.subjectSelenocysteine
dc.subjectselD
dc.subjectLeishmania
dc.subjectTrypanosoma
dc.titleSelenocysteine incorporation in Kinetoplastid: Selenophosphate synthetase (SELD) from Leishmania major and Trypanosoma brucei
dc.typeArtículos de revistas


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