dc.creatorCAVALCANTE, R. S.
dc.creatorIMASATO, H.
dc.creatorBagnato, Vanderlei Salvador
dc.creatorPERUSSI, J. R.
dc.date.accessioned2012-10-20T04:16:23Z
dc.date.accessioned2018-07-04T15:42:16Z
dc.date.available2012-10-20T04:16:23Z
dc.date.available2018-07-04T15:42:16Z
dc.date.created2012-10-20T04:16:23Z
dc.date.issued2009
dc.identifierLASER PHYSICS LETTERS, v.6, n.1, p.64-70, 2009
dc.identifier1612-2011
dc.identifierhttp://producao.usp.br/handle/BDPI/29738
dc.identifier10.1002/lapl.200810082
dc.identifierhttp://dx.doi.org/10.1002/lapl.200810082
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1626378
dc.description.abstractPhotodynamic therapy, used mainly for cancer treatment and microorganisms inaction, is based on production of reactive oxygen species by light irradiation of a sensitizer. Hematoporphyrin derivatives as Photofrin (R) (PF) Photogem (R) (PG) and Photosan (R) (PF), and chlorin-c6-derivatives as Photodithazine (R)(PZ), have suitable sensitizing properties. The present study provides a way to make a fast previous evaluation of photosensitizers efficacy by a combination of techniques: a) use of brovine serum albumin and uric acid as chemical dosimeters; b) photo-hemolysis of red blood cells used as a cell membrane interaction model, and c) octanol/phosphate buffer partition to assess the relative lipophilicity of the compounds. The results suggest the photodynamic efficient rankings PZ > PG >= PF > PS. These results agree with the cytotoxicity of the photosensitizers as well as to chromatographic separation of the HpDs, both performed in our group, showing that the more lipophilic is the dye, the more acute is the damage to the RBC membrane and the oxidation of indol, which is immersed in the hydrophobic region of albumin.
dc.languageeng
dc.publisherWILEY-V C H VERLAG GMBH
dc.relationLaser Physics Letters
dc.rightsCopyright WILEY-V C H VERLAG GMBH
dc.rightsrestrictedAccess
dc.subjectphotodynamic therapy
dc.subjecthematoporphyrin derivatives
dc.subjectchlorin derivative
dc.subjectphotodynamic efficiency
dc.subjectphoto-hemolysis
dc.subjectalbumin
dc.titleA combination of techniques to evaluate photodynamic efficiency of photosensitizers
dc.typeArtículos de revistas


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