Use of PCR for detecting Aspergillus flavus in maize treated by gamma radiation process

dc.creatorAQUINO, Simone
dc.creatorGREINER, Ralf
dc.creatorKONIETZNY, Ursula
dc.creatorHASSEGAWA, Regina H.
dc.creatorREIS, Tatiana Alves dos
dc.creatorCORREA, Benedito
dc.creatorVILLAVICENCIO, Anna Lucia C. H.
dc.date.accessioned2012-10-20T03:24:26Z
dc.date.accessioned2018-07-04T15:36:12Z
dc.date.available2012-10-20T03:24:26Z
dc.date.available2018-07-04T15:36:12Z
dc.date.created2012-10-20T03:24:26Z
dc.date.issued2008
dc.identifierFOOD BIOTECHNOLOGY, v.22, n.1/Fev, p.150-159, 2008
dc.identifier0890-5436
dc.identifierhttp://producao.usp.br/handle/BDPI/28381
dc.identifier10.1080/08905430802043164
dc.identifierhttp://dx.doi.org/10.1080/08905430802043164
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1625024
dc.description.abstractThe aim of this study was to verify the effects of gamma radiation process on the fungal DNA and the application of PCR in the detection of Aspergillus flavus in irradiated maize grains. The samples were inoculated with a toxigenic strain and incubated under controlled conditions of relative humidity, water activity, and temperature for 15 days. After incubation, the samples were treated with gamma radiation with doses of 5 and 10 kGy and individually analyzed. The use of PCR technique showed the presence of DNA bands of Aspergillus flavus in all irradiated samples that showed no fungal growth in agar medium.
dc.languageeng
dc.publisherTAYLOR & FRANCIS INC
dc.relationFood Biotechnology
dc.rightsCopyright TAYLOR & FRANCIS INC
dc.rightsrestrictedAccess
dc.subjectgamma radiation
dc.subjectaspergillus flavus
dc.subjectaflatoxin
dc.subjectmaize
dc.subjectPCR
dc.subjectDNA
dc.titleUse of PCR for detecting Aspergillus flavus in maize treated by gamma radiation process
dc.typeArtículos de revistas


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