dc.creatorSEIBERT, Carla Simone
dc.creatorSANTORO, Marcelo L.
dc.creatorTAMBOURGI, Denise Vilarinho
dc.creatorSAMPAIO, Sandra Coccuzzo
dc.creatorTAKAHASHI, Hilton Kenji
dc.creatorPERES, Carmem Maldonado
dc.creatorCuri, Rui
dc.creatorSANO-MARTINS, Ida S.
dc.date.accessioned2012-10-20T03:18:27Z
dc.date.accessioned2018-07-04T15:34:39Z
dc.date.available2012-10-20T03:18:27Z
dc.date.available2018-07-04T15:34:39Z
dc.date.created2012-10-20T03:18:27Z
dc.date.issued2010
dc.identifierTOXICON, v.55, n.7, p.1323-1330, 2010
dc.identifier0041-0101
dc.identifierhttp://producao.usp.br/handle/BDPI/28027
dc.identifier10.1016/j.toxicon.2010.02.003
dc.identifierhttp://dx.doi.org/10.1016/j.toxicon.2010.02.003
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1624671
dc.description.abstractLonomia obliqua caterpillar bristle extract induces hemolysis in vitro on washed human and rat erythrocytes, in either the absence or presence of exogenous lecithin. In the former condition, phospholipases A(2) are key enzymes involved in hemolysis. However, the mechanism whereby this extract causes direct hemolysis is not known. Thus, the aim of this study was to investigate the hemolytic mechanism of the crude extract of the caterpillar L obliqua on human erythrocytes in the absence of lecithin. The extract significantly increased the erythrocyte osmotic fragility and promoted the removal of glycophorins A and C, and band 3 from the erythrocyte membrane. The use of Ca(2+) and Mg(2+) ions significantly potentiated glycoprotein removal, remarkably of erythrocyte band 3. The composition of fatty acids was analyzed by HPLC in both L obliqua caterpillar bristle extract and human erythrocyte membranes incubated with the extract. The levels of unsaturated fatty acids were remarkably augmented in erythrocytes incubated with the extract than in control erythrocytes, modifying thereby the saturated/unsaturated fatty acid ratio. Altogether, evidence is provided here that the interplay of at least three mechanisms of action accounts for the direct activity of the bristle extract on erythrocyte membrane, leading to hemolysis: the removal of glycoproteins and band 3; the insertion of fatty acids; and the action of phospholipases. Such mechanisms might affect erythrocyte flexibility and deformability, which may induce hemolysis by increasing erythrocyte fragility. However, whether the direct hemolytic activity of L obliqua caterpillar is the major cause of intravascular hemolysis during envenomation still needs further investigation. (C) 2010 Elsevier Ltd. All rights reserved.
dc.languageeng
dc.publisherPERGAMON-ELSEVIER SCIENCE LTD
dc.relationToxicon
dc.rightsCopyright PERGAMON-ELSEVIER SCIENCE LTD
dc.rightsrestrictedAccess
dc.subjectBand 3
dc.subjectChromatography
dc.subjectFatty acids
dc.subjectGlycophorin
dc.subjectLipids
dc.subjectPhospholipase A(2)
dc.titleLonomia obliqua (Lepidoptera, Saturniidae) caterpillar bristle extract induces direct lysis by cleaving erythrocyte membrane glycoproteins
dc.typeArtículos de revistas


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