Artículos de revistas
The plasmodium receptor for activated C kinase protein inhibits Ca(2+) signaling in mammalian cells
Fecha
2009Registro en:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.389, n.4, p.586-592, 2009
0006-291X
10.1016/j.bbrc.2009.09.025
Autor
SARTORELLO, Robson
AMAYA, Maria Jimena
NATHANSON, Michael H.
GARCIA, Celia R. S.
Institución
Resumen
Plasmodium falciparum, the most lethal malarial parasite, expresses an ortholog for the protein kinase C (PKC) activator RACK1. However, PKC has not been identified in this parasite, and the mammalian RACK1 can interact with the inositol 1,4,5-trisphosphate receptor (InsP3R). Therefore we investigated whether the Plasmodium ortholog PfRACK also can affect InsP3R-mediated Ca(2+) signaling in mammalian cells. GFP-tagged PfRACK and endogenous RACK1 were expressed in a similar distribution within cells. PfRACK inhibited agonist-induced Ca(2+) signals in cells expressing each isoform of the InsP3R, and this effect persisted when expression of endogenous RACK1 was reduced by siRNA. PfRACK also inhibited Ca(2+) signals induced by photorelease of caged InsP3. These findings provide evidence that PfRACK directly inhibits InsP3-mediated Ca(2+) signaling in mammalian cells. Interference with host cell signaling pathways to subvert the host intracellular milieu may be an important mechanism for parasite survival. (C) 2009 Elsevier Inc. All rights reserved.