Artículos de revistas
Trigeminal nitric oxide synthase expression correlates with new bone formation during distraction osteogenesis
Fecha
2008Registro en:
CALCIFIED TISSUE INTERNATIONAL, v.82, n.4, p.309-315, 2008
0171-967X
10.1007/s00223-008-9107-8
Autor
ALBUQUERQUE JR., Rubens Ferreira de
BEL, Elaine Aparecida Del
BRENTEGANI, Luiz Guilherme
OLIVEIRA, Maria Tereza Moura de
ISSA, Joao Paulo Mardegan
Institución
Resumen
Nitric oxide synthase (NOS) has been reported to be involved with both bone healing and bone metabolism. The aim of this study was to test the null hypothesis that there is no correlation between new bone formation during mandibular distraction osteogenesis and NOS expression in the trigeminal ganglion of rats. Newly formed tissue during distraction osteogenesis and trigeminal NOS expression measured by the NADPH-diaphorase (NADPH-d) reaction were evaluated in 72 male Wistar rats by histomorphometric and histochemical methods. In animals submitted to 0.5 mm/day distraction osteogenesis, the percentage of bone tissue was higher in the basal area of the mandibles compared with the center and significantly increased through the experimental periods (P < 0.05). At the sixth postoperative week, the difference in bone formation between the continuous and acute distraction osteogenesis groups was the highest. Significant correlation between new bone formation by distraction osteogenesis and NADPH-d-reactive neurons was found, varying according to neuronal cell size (r = -0.6, P = 0.005, small cells strongly stained; r = 0.5, P = 0.018, large cells moderately stained). The results suggest that NOS may play a role in the bone healing process via neurogenic pathways, and the phenomenon seems to be neuronal cell morphotype-dependent. Further studies are now warranted to investigate the mechanistic link between the expression of trigeminal NOS and mandibular new bone formation by distraction osteogenesis.