dc.creatorOLIVEIRA, Paulo Tambasco de
dc.creatorOLIVA, Marcos Andrade de
dc.creatorMAXIMIANO, William Marcatti Amaru
dc.creatorSEBASTIAO, Karen Elaine Vasconcelos
dc.creatorCRIPPA, Grasiele Edilaine
dc.creatorCIANCAGLINI, Pietro
dc.creatorBELOTI, Marcio Mateus
dc.creatorNANCI, Antonio
dc.creatorROSA, Adalberto Luiz
dc.date.accessioned2012-10-20T01:15:46Z
dc.date.accessioned2018-07-04T15:26:15Z
dc.date.available2012-10-20T01:15:46Z
dc.date.available2018-07-04T15:26:15Z
dc.date.created2012-10-20T01:15:46Z
dc.date.issued2008
dc.identifierJOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, v.56, n.7, p.629-638, 2008
dc.identifier0022-1554
dc.identifierhttp://producao.usp.br/handle/BDPI/26280
dc.identifier10.1369/jhc.2008.950758
dc.identifierhttp://dx.doi.org/10.1369/jhc.2008.950758
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1622944
dc.description.abstractStrategies to promote bone repair have included exposure of cells to growth factor (GF) preparations from blood that generally include proteins as part of a complex mixture. This study aimed to evaluate the effects of such a mixture on different parameters of the development of the osteogenic phenotype in vitro. Osteoblastic cells were obtained by enzymatic digestion of human alveolar bone and cultured under standard osteogenic conditions until subconfluence. They were subcultured on Thermanox coverslips up to 14 days. Treated cultures were exposed during the first 7 days to osteogenic medium supplemented with a GFs + proteins mixture containing the major components found in platelet extracts [plate I et-derived growth factor-BB, transforming growth factor (TGF)-beta 1, TGF-beta 2, albumin, fibronectin, and thrombospondin] and to osteogenic medium alone thereafter. Control cultures were exposed only to the osteogenic medium. Treated cultures exhibited a significantly higher number of adherent cells from day 4 onward and of cycling cells at days 1 and 4, weak alkaline phosphatase (ALP) labeling, and significantly decreased levels of ALP activity and mRNA expression. At day 14, no Alizarin red-stained nodular areas were detected in cultures treated with GFs + proteins. Results were confirmed in the rat calvaria-derived osteogenic cell culture model. The addition of bone morphogenetic protein 7 or growth and differentiation factor 5 to treated cultures upregulated Runx2 and ALP mRNA expression, but surprisingly, ALP activity was not restored. These results showed that a mixture of GFs + proteins affects the development of the osteogenic phenotype both in human and rat cultures, leading to an increase in the number of cells, but expressed a less differentiated state.
dc.languageeng
dc.publisherHISTOCHEMICAL SOC INC
dc.relationJournal of Histochemistry & Cytochemistry
dc.rightsCopyright HISTOCHEMICAL SOC INC
dc.rightsrestrictedAccess
dc.subjectcell culture
dc.subjectosteoblasts
dc.subjectgrowth factors
dc.subjectcell proliferation
dc.subjectalkaline phosphatase
dc.subjectmineralization
dc.titleEffects of a mixture of growth factors and proteins on the development of the osteogenic phenotype in human alveolar bone cell cultures
dc.typeArtículos de revistas


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