dc.creator | OLIVEIRA, Gabriela Pintar de | |
dc.creator | MAXIMINO, Jessica Ruivo | |
dc.creator | LIN, Chin Jia | |
dc.creator | CHADI, Gerson | |
dc.date.accessioned | 2012-10-19T17:30:28Z | |
dc.date.accessioned | 2018-07-04T15:07:55Z | |
dc.date.available | 2012-10-19T17:30:28Z | |
dc.date.available | 2018-07-04T15:07:55Z | |
dc.date.created | 2012-10-19T17:30:28Z | |
dc.date.issued | 2009 | |
dc.identifier | JOURNAL OF MOLECULAR HISTOLOGY, v.40, n.3, p.217-225, 2009 | |
dc.identifier | 1567-2379 | |
dc.identifier | http://producao.usp.br/handle/BDPI/22237 | |
dc.identifier | 10.1007/s10735-009-9233-2 | |
dc.identifier | http://dx.doi.org/10.1007/s10735-009-9233-2 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1619010 | |
dc.description.abstract | Neuron-glia interaction is involved in physiological function of neurons, however, recent evidences have suggested glial cells as participants in neurotoxic and neurotrophic mechanisms of neurodegenerative/neuroregenerative processes. Laser microdissection offers a unique opportunity to study molecular regulation in specific immunolabeled cell types. However, an adequate protocol to allow morphological and molecular analysis of rodent spinal cord astrocyte, microglia and motoneurons remains a big challenge. In this paper we present a quick method to immunolabel those cells in flash frozen sections to be used in molecular biology analyses after laser microdissection and pressure catapulting. | |
dc.language | eng | |
dc.publisher | SPRINGER | |
dc.relation | Journal of Molecular Histology | |
dc.rights | Copyright SPRINGER | |
dc.rights | restrictedAccess | |
dc.subject | Astrocytes | |
dc.subject | Cell microdissection | |
dc.subject | Method | |
dc.subject | Microglia | |
dc.subject | Motoneuron | |
dc.subject | Review | |
dc.title | A method to immunolabel rodent spinal cord neurons and glia for molecular study in specific laser microdissected cells involved in neurodegenerative disorders | |
dc.type | Artículos de revistas | |