dc.creatorCUNHA, J. P. C. da
dc.creatorGALANTE, P. A. F.
dc.creatorSOUZA, J. E. de
dc.creatorSOUZA, R. F. de
dc.creatorCARVALHO, P. M.
dc.creatorOHARA, D. T.
dc.creatorMOURA, R. P.
dc.creatorOBA-SHINJA, S. M.
dc.creatorMARIE, S. K. N.
dc.creatorSILVA JR., W. A.
dc.creatorPEREZ, R. O.
dc.creatorSTRANSKY, B.
dc.creatorPIEPRZYK, M.
dc.creatorMOORE, J.
dc.creatorCABALLERO, O.
dc.creatorGAMA-RODRIGUES, J.
dc.creatorHABR-GAMA, A.
dc.creatorKUO, W. P.
dc.creatorSIMPSON, A. J.
dc.creatorCAMARGO, A. A.
dc.creatorOLD, Lloyd J.
dc.creatorSOUZA, S. J. de
dc.date.accessioned2012-10-19T17:30:28Z
dc.date.accessioned2018-07-04T15:07:55Z
dc.date.available2012-10-19T17:30:28Z
dc.date.available2018-07-04T15:07:55Z
dc.date.created2012-10-19T17:30:28Z
dc.date.issued2009
dc.identifierPROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.106, n.39, p.16752-16757, 2009
dc.identifier0027-8424
dc.identifierhttp://producao.usp.br/handle/BDPI/22235
dc.identifier10.1073/pnas.0907939106
dc.identifierhttp://dx.doi.org/10.1073/pnas.0907939106
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1619008
dc.description.abstractCell surface proteins are excellent targets for diagnostic and therapeutic interventions. By using bioinformatics tools, we generated a catalog of 3,702 transmembrane proteins located at the surface of human cells (human cell surfaceome). We explored the genetic diversity of the human cell surfaceome at different levels, including the distribution of polymorphisms, conservation among eukaryotic species, and patterns of gene expression. By integrating expression information from a variety of sources, we were able to identify surfaceome genes with a restricted expression in normal tissues and/or differential expression in tumors, important characteristics for putative tumor targets. A high-throughput and efficient quantitative real-time PCR approach was used to validate 593 surfaceome genes selected on the basis of their expression pattern in normal and tumor samples. A number of candidates were identified as potential diagnostic and therapeutic targets for colorectal tumors and glioblastoma. Several candidate genes were also identified as coding for cell surface cancer/testis antigens. The human cell surfaceome will serve as a reference for further studies aimed at characterizing tumor targets at the surface of human cells.
dc.languageeng
dc.publisherNATL ACAD SCIENCES
dc.relationProceedings of the National Academy of Sciences of the United States of America
dc.rightsCopyright NATL ACAD SCIENCES
dc.rightsrestrictedAccess
dc.subjectcolorectal tumors
dc.subjectCT antigens
dc.subjectglioblastoma
dc.subjecttransmembrane
dc.subjecttumor cell surface antigens
dc.titleBioinformatics construction of the human cell surfaceome
dc.typeArtículos de revistas


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