dc.creator | OLIVEIRA, Claudia Pinto Marques Souza | |
dc.creator | STEFANO, Jose Tadeu | |
dc.creator | CAVALEIRO, Ana Mercedes | |
dc.creator | FORTES, Maria Angela Henriques Zanella | |
dc.creator | VIEIRA, Suzana Maria | |
dc.creator | LIMA, Vicencia Mara Rodrigues | |
dc.creator | SANTOS, Telma Eugenio | |
dc.creator | SANTOS, Virginia Nascimento | |
dc.creator | SALGADO, Ana Lucia Farias de Azevedo | |
dc.creator | PARISE, Edson Roberto | |
dc.creator | ALVES, Venancio Avancini Ferreira | |
dc.creator | CARRILHO, Flair Jose | |
dc.creator | CORREA-GIANNELLA, Maria Lucia | |
dc.date.accessioned | 2012-10-19T17:25:22Z | |
dc.date.accessioned | 2018-07-04T15:07:20Z | |
dc.date.available | 2012-10-19T17:25:22Z | |
dc.date.available | 2018-07-04T15:07:20Z | |
dc.date.created | 2012-10-19T17:25:22Z | |
dc.date.issued | 2010 | |
dc.identifier | JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY, v.25, n.2, p.357-361, 2010 | |
dc.identifier | 0815-9319 | |
dc.identifier | http://producao.usp.br/handle/BDPI/22100 | |
dc.identifier | 10.1111/j.1440-1746.2009.06001.x | |
dc.identifier | http://dx.doi.org/10.1111/j.1440-1746.2009.06001.x | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1618873 | |
dc.description.abstract | Background and Aims: Although the metabolic risk factors for non-alcoholic fatty liver disease (NAFLD) progression have been recognized, the role of genetic susceptibility remains a field to be explored. The aim of this study was to examine the frequency of two polymorphisms in Brazilian patients with biopsy-proven simple steatosis or non-alcoholic steatohepatitis (NASH): -493 G/T in the MTP gene, which codes the protein responsible for transferring triglycerides to nascent apolipoprotein B, and -129 C/T in the GCLC gene, which codes the catalytic subunit of glutamate-cystein ligase in the formation of glutathione. Methods: One hundred and thirty-one biopsy-proven NAFLD patients (n = 45, simple steatosis; n = 86, NASH) and 141 unrelated healthy volunteers were evaluated. Genomic DNA was extracted from peripheral blood cells, and the -129 C/T polymorphism of the GCLC gene was determined by restriction fragment length polymorphism (RFLP). The -493 G/T polymorphism of the MTP gene was determined by direct sequencing of the polymerase chain reaction products. Results: The presence of at least one T allele in the -129 C/T polymorphism of the GCLC gene was independently associated with NASH (odds ratio 12.14, 95% confidence interval 2.01-73.35; P = 0.007), whereas, the presence of at least one G allele in the -493 G/T polymorphism of the MTP gene differed slightly between biopsy-proven NASH and simple steatosis. Conclusion: This difference clearly warrants further investigation in larger samples. These two polymorphisms could represent an additional factor for consideration in evaluating the risk of NAFLD progression. Further studies involving a larger population are necessary to confirm this notion. | |
dc.language | eng | |
dc.publisher | WILEY-BLACKWELL PUBLISHING, INC | |
dc.relation | Journal of Gastroenterology and Hepatology | |
dc.rights | Copyright WILEY-BLACKWELL PUBLISHING, INC | |
dc.rights | restrictedAccess | |
dc.subject | fatty liver | |
dc.subject | genetic susceptibility | |
dc.subject | genetic polymorphisms | |
dc.subject | microsomal TG transfer protein | |
dc.subject | lipid metabolism | |
dc.subject | glutamate-cystein ligase | |
dc.subject | glutathione | |
dc.subject | oxidative stress | |
dc.title | Association of polymorphisms of glutamate-cystein ligase and microsomal triglyceride transfer protein genes in non-alcoholic fatty liver disease | |
dc.type | Artículos de revistas | |