dc.creatorSOMERA, Alexandre Favarin
dc.creatorPEREIRA, Marita Gimenez
dc.creatorGUIMARAES, Luis Henrique Souza
dc.creatorPOLIZELI, Maria de Lourdes Teixeira de Moraes
dc.creatorTERENZI, Hector Francisco
dc.creatorFURRIEL, Rosa Prazeres Melo
dc.creatorJORGE, Joao Atilio
dc.date.accessioned2012-10-19T14:13:06Z
dc.date.accessioned2018-07-04T15:00:23Z
dc.date.available2012-10-19T14:13:06Z
dc.date.available2018-07-04T15:00:23Z
dc.date.created2012-10-19T14:13:06Z
dc.date.issued2009
dc.identifierJOURNAL OF MICROBIOLOGY, v.47, n.3, p.270-276, 2009
dc.identifier1225-8873
dc.identifierhttp://producao.usp.br/handle/BDPI/20676
dc.identifier10.1007/s12275-008-0286-9
dc.identifierhttp://dx.doi.org/10.1007/s12275-008-0286-9
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1617455
dc.description.abstractAspergillus versicolor grown on xylan or xylose produces two beta-xylosidases with differences in biochemical properties and degree of glycosylation. We investigated the alterations in the biochemical properties of these beta-xylosidases after deglycosylation with Endo-H or PNGase F. After deglycosylation, both enzymes migrated faster in PAGE or SDS-PAGE exhibiting the same R(f). Temperature optimum of xylan-induced and xylose-induced beta-xylosidases was 45A degrees C and 40A degrees C, respectively, and 35A degrees C after deglycosylation. The xylan-induced enzyme was more active at acidic pH. After deglycosylation, both enzymes had the same pH optimum of 6.0. Thermal resistance at 55A degrees C showed half-life of 15 min and 9 min for xylose- and xylan-induced enzymes, respectively. After deglycosylation, both enzymes exhibited half-lives of 7.5 min. Native enzymes exhibited different responses to ions, while deglycosylated enzymes exhibited identical responses. Limited proteolysis yielded similar polypeptide profiles for the deglycosylated enzymes, suggesting a common polypeptide core with differential glycosylation apparently responsible for their biochemical and biophysical differences.
dc.languageeng
dc.publisherMICROBIOLOGICAL SOCIETY KOREA
dc.relationJournal of Microbiology
dc.rightsCopyright MICROBIOLOGICAL SOCIETY KOREA
dc.rightsrestrictedAccess
dc.subjectbeta-xylosidase
dc.subjectxylobiase
dc.subjectglycosylation
dc.subjectdeglycosylation
dc.subjectglycoprotein
dc.subjectAspergillus versicolor
dc.titleEffect of glycosylation on the biochemical properties of beta-xylosidases from Aspergillus versicolor
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución