dc.creatorMALAVAZI, Iran
dc.creatorLIMA, Joel Fernandes
dc.creatorCASTRO, Patricia Alves de
dc.creatorSAVOLDI, Marcela
dc.creatorGOLDMANT, Maria Helena de Souza
dc.creatorGOLDMAN, Gustavo Henrique
dc.date.accessioned2012-10-19T14:12:37Z
dc.date.accessioned2018-07-04T15:00:03Z
dc.date.available2012-10-19T14:12:37Z
dc.date.available2018-07-04T15:00:03Z
dc.date.created2012-10-19T14:12:37Z
dc.date.issued2008
dc.identifierGENETICS, v.178, n.2, p.675-691, 2008
dc.identifier0016-6731
dc.identifierhttp://producao.usp.br/handle/BDPI/20599
dc.identifier10.1534/genetics.107.080879
dc.identifierhttp://dx.doi.org/10.1534/genetics.107.080879
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1617381
dc.description.abstractAtaxia telangiectasia mutated (ATM) is a phosphatidyl-3-kinase-related protein kinase that functions as a central regulator of the DNA damage response in eukaryotic cells. In humans, mutations in ATM cause the devastating neurodegenerative disease ataxia telangiectasia. Previously, we characterized the homolog of ATM (AtmA) in the filamentous fungus Aspergillus nidulans. In addition to its expected role in the DNA damage response, we found that AtmA is also required for polarized hyphal growth. Here, we extended these studies by investigating which components of the DNA damage response pathway are interacting with AtmA. The AtmA(ATM) loss of function caused synthetic lethality when combined with mutation in UvsB(ATR). Our results suggest that AtmA and UvsB are interacting and they are probably partially redundant in terms of DNA damage sensing and/or repairing and polar growth. We identified and inactivated A. nidulans chkA(CHK1) and chkB(CHK2) genes. These genes are also redundantly involved in A. nidulans DNA damage response. We constructed several combinations of double mutants for Delta atmA, Delta uvsB, Delta chkA, and Delta chkB. We observed a complex genetic relationship with these mutations during the DNA replication checkpoint and DNA damage response. Finally, we observed epistatic and synergistic interactions between AtmA, and bimE(APCI), ankA(WEE1) and the cdc2-related kinase npkA, at S-phase checkpoint and in response to DNA-damaging agents.
dc.languageeng
dc.publisherGENETICS
dc.relationGenetics
dc.rightsCopyright GENETICS
dc.rightsrestrictedAccess
dc.titleGenetic interactions of the Ashergillus nidulans atmA(ATM) homolog with different components of the DNA damage response pathway
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución