dc.creatorMAGALHAES, Igor R. S.
dc.creatorJABOR, Valquiria A. P.
dc.creatorFARIA, Anizio M.
dc.creatorCOLLINS, Carol H.
dc.creatorJARDIM, Isabel C. S. F.
dc.creatorBONATO, Pierina S.
dc.date.accessioned2012-10-19T03:40:24Z
dc.date.accessioned2018-07-04T14:57:27Z
dc.date.available2012-10-19T03:40:24Z
dc.date.available2018-07-04T14:57:27Z
dc.date.created2012-10-19T03:40:24Z
dc.date.issued2010
dc.identifierTALANTA, v.81, n.3, p.941-947, 2010
dc.identifier0039-9140
dc.identifierhttp://producao.usp.br/handle/BDPI/20004
dc.identifier10.1016/j.talanta.2010.01.039
dc.identifierhttp://dx.doi.org/10.1016/j.talanta.2010.01.039
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1616788
dc.description.abstractA method for the determination of artemether (ART) and its main metabolite dihydroartemisinin (DHA) in plasma employing liquid-phase microextraction (LPME) for sample preparation prior to liquid chromatography-tandem mass spectrometry (LC-MS-MS) was developed. The analytes were extracted from 1 nil, of plasma utilizing a two-phase LPME procedure with artemisinin as internal standard. Using the optimized LPME conditions, mean absolute recovery rates of 25 and 32% for DHA and ART, respectively, were achieved using toluene-n-octanol (1:1, viv) as organic phase with an extraction time of 30 min. After extraction, the analytes were resolved within 5 min using a mobile phase consisting of methanol-ammonium acetate (10 mmol L(-1) pH 5.0, 80:20. v/v) on a laboratory-made column based on poly(methyltetradecylsiloxane) attached to a zirconized-silica support. MS-MS detection was employed using an electrospray interface in the positive ion mode. The method developed was linear over the range of 5-1000 ng mL(-1) for both analytes. Precision and accuracy were within acceptable levels of confidence (<15%). The assay was applied to the determination of these analytes in plasma from rats treated with ART. The two-phase LPME procedure is affordable and the solvent consumption was very low compared to the traditional methods of sample preparation. (C) 2010 Elsevier B.V. All rights reserved.
dc.languageeng
dc.publisherELSEVIER SCIENCE BV
dc.relationTalanta
dc.rightsCopyright ELSEVIER SCIENCE BV
dc.rightsrestrictedAccess
dc.subjectArtemether
dc.subjectDihydroartemisinin
dc.subjectLiquid-phase microextraction
dc.subjectLiquid chromatography-mass spectrometry
dc.subjectPlasma
dc.titleDetermination of beta-artemether and its main metabolite dihydroartemisinin in plasma employing liquid-phase microextraction prior to liquid chromatographic-tandem mass spectrometric analysis
dc.typeArtículos de revistas


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