dc.creator | MAGALHAES, Igor R. S. | |
dc.creator | JABOR, Valquiria A. P. | |
dc.creator | FARIA, Anizio M. | |
dc.creator | COLLINS, Carol H. | |
dc.creator | JARDIM, Isabel C. S. F. | |
dc.creator | BONATO, Pierina S. | |
dc.date.accessioned | 2012-10-19T03:40:24Z | |
dc.date.accessioned | 2018-07-04T14:57:27Z | |
dc.date.available | 2012-10-19T03:40:24Z | |
dc.date.available | 2018-07-04T14:57:27Z | |
dc.date.created | 2012-10-19T03:40:24Z | |
dc.date.issued | 2010 | |
dc.identifier | TALANTA, v.81, n.3, p.941-947, 2010 | |
dc.identifier | 0039-9140 | |
dc.identifier | http://producao.usp.br/handle/BDPI/20004 | |
dc.identifier | 10.1016/j.talanta.2010.01.039 | |
dc.identifier | http://dx.doi.org/10.1016/j.talanta.2010.01.039 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1616788 | |
dc.description.abstract | A method for the determination of artemether (ART) and its main metabolite dihydroartemisinin (DHA) in plasma employing liquid-phase microextraction (LPME) for sample preparation prior to liquid chromatography-tandem mass spectrometry (LC-MS-MS) was developed. The analytes were extracted from 1 nil, of plasma utilizing a two-phase LPME procedure with artemisinin as internal standard. Using the optimized LPME conditions, mean absolute recovery rates of 25 and 32% for DHA and ART, respectively, were achieved using toluene-n-octanol (1:1, viv) as organic phase with an extraction time of 30 min. After extraction, the analytes were resolved within 5 min using a mobile phase consisting of methanol-ammonium acetate (10 mmol L(-1) pH 5.0, 80:20. v/v) on a laboratory-made column based on poly(methyltetradecylsiloxane) attached to a zirconized-silica support. MS-MS detection was employed using an electrospray interface in the positive ion mode. The method developed was linear over the range of 5-1000 ng mL(-1) for both analytes. Precision and accuracy were within acceptable levels of confidence (<15%). The assay was applied to the determination of these analytes in plasma from rats treated with ART. The two-phase LPME procedure is affordable and the solvent consumption was very low compared to the traditional methods of sample preparation. (C) 2010 Elsevier B.V. All rights reserved. | |
dc.language | eng | |
dc.publisher | ELSEVIER SCIENCE BV | |
dc.relation | Talanta | |
dc.rights | Copyright ELSEVIER SCIENCE BV | |
dc.rights | restrictedAccess | |
dc.subject | Artemether | |
dc.subject | Dihydroartemisinin | |
dc.subject | Liquid-phase microextraction | |
dc.subject | Liquid chromatography-mass spectrometry | |
dc.subject | Plasma | |
dc.title | Determination of beta-artemether and its main metabolite dihydroartemisinin in plasma employing liquid-phase microextraction prior to liquid chromatographic-tandem mass spectrometric analysis | |
dc.type | Artículos de revistas | |