Cytotoxic activity of a dichloromethane extract and fractions obtained from Eudistoma vannamei (Tunicata: Ascidiacea)

dc.creatorJIMENEZ, Paula C.
dc.creatorWILKE, Diego V.
dc.creatorTAKEARA, Renata
dc.creatorLOTUFO, Tito M. C.
dc.creatorPESSOA, Claudia
dc.creatorMORAES, Manoel Odorico de
dc.creatorLOPES, Norberto P.
dc.creatorCOSTA-LOTUFO, Leticia V.
dc.date.accessioned2012-10-19T03:39:54Z
dc.date.accessioned2018-07-04T14:57:06Z
dc.date.available2012-10-19T03:39:54Z
dc.date.available2018-07-04T14:57:06Z
dc.date.created2012-10-19T03:39:54Z
dc.date.issued2008
dc.identifierCOMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, v.151, n.3, Special Issue, p.391-398, 2008
dc.identifier1095-6433
dc.identifierhttp://producao.usp.br/handle/BDPI/19926
dc.identifier10.1016/j.cbpa.2007.02.018
dc.identifierhttp://dx.doi.org/10.1016/j.cbpa.2007.02.018
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1616711
dc.description.abstractThis study consists of the bioassay-guided fractionation of the dichloromethane extract from Eudistoma vannamei and the pharmacological characterization of the active fractions. The dried hydromethanolic extract dissolved in aqueous methanol was partitioned with dichloromethane and chromatographed on a silica gel flash column. The anti-proliferative effect was monitored by the MTT assay. Four of the latest fractions, numbered 14 to 17, which held many chemical similarities amongst each other, were found to be the most active. The selected fractions were tested for viability, proliferation and death induction on cultures of HL-60 promycloblastic leukemia cells. The results suggested that the observed cytotoxicity is related to apoptosis induction. (C) 2007 Elsevier Inc. All rights reserved.
dc.languageeng
dc.publisherELSEVIER SCIENCE INC
dc.relationComparative Biochemistry and Physiology A-molecular & Integrative Physiology
dc.rightsCopyright ELSEVIER SCIENCE INC
dc.rightsrestrictedAccess
dc.subjectAscidians
dc.subjectApoptosis
dc.subjectCyotoxicity
dc.subjectEudistoma vannamei
dc.subjectMTT assay
dc.subjecttumor cell lines
dc.titleCytotoxic activity of a dichloromethane extract and fractions obtained from Eudistoma vannamei (Tunicata: Ascidiacea)
dc.typeArtículos de revistas


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