dc.creator | BEZERRA, Raquel Pedrosa | |
dc.creator | MATSUDO, Marcelo Chuei | |
dc.creator | CONVERTI, Attilio | |
dc.creator | Sato, Sunao | |
dc.creator | CARVALHO, Joao Carlos Monteiro de | |
dc.date.accessioned | 2012-10-19T03:18:26Z | |
dc.date.accessioned | 2018-07-04T14:56:32Z | |
dc.date.available | 2012-10-19T03:18:26Z | |
dc.date.available | 2018-07-04T14:56:32Z | |
dc.date.created | 2012-10-19T03:18:26Z | |
dc.date.issued | 2008 | |
dc.identifier | BIOTECHNOLOGY AND BIOENGINEERING, v.100, n.2, p.297-305, 2008 | |
dc.identifier | 0006-3592 | |
dc.identifier | http://producao.usp.br/handle/BDPI/19800 | |
dc.identifier | 10.1002/bit.21771 | |
dc.identifier | http://dx.doi.org/10.1002/bit.21771 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1616585 | |
dc.description.abstract | This study dealt with the influence of both the feeding time and light intensity on the fed-batch culture of the cyanobacterium Spirulina (Arthrospira) platensis using ammonium chloride as a nitrogen source. For this purpose, a 2 2 plus star central composite experimental design combined with response surface methodology was employed, and the maximum cell concentration (X-m), the cell productivity (P-X), and the yield of biomass on nitrogen (Y-X/N) were selected as the response variables. The optimum values of X-m (1,833 mgL(-1)) and Y-X/N (5.9 gg(-1)) estimated by the model at light intensity of 13 klux and feeding time of 17.2 days were very close to those obtained experimentally under these conditions (X-m = 1,771 +/- 41 mg L-1; Y-X/N = 5.7 +/- 0.17 gg(-1)). The cell productivity was a decreasing function of the ammonium chloride feeding time and a quadratic function of the light intensity. The protein and lipid contents of dry biomass collected at the end of cultivations were shown to decrease with increasing light intensity. | |
dc.language | eng | |
dc.publisher | JOHN WILEY & SONS INC | |
dc.relation | Biotechnology and Bioengineering | |
dc.rights | Copyright JOHN WILEY & SONS INC | |
dc.rights | restrictedAccess | |
dc.subject | ammonium chloride | |
dc.subject | light intensity | |
dc.subject | Spirulina (Arthrospira) platensis | |
dc.subject | fed-batch cultivation | |
dc.subject | response surface methodology | |
dc.title | Influence of ammonium chloride feeding time and light intensity on the cultivation of Spirulina (Arthrospira) platensis | |
dc.type | Artículos de revistas | |