Artículos de revistas
Bacteriosomes in axenic plants: endophytes as stable endosymbionts
Fecha
2009Registro en:
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, v.25, n.10, p.1757-1764, 2009
0959-3993
10.1007/s11274-009-0073-8
Autor
ALMEIDA, Cristina Vieira de
ANDREOTE, Fernando Dini
YARA, Ricardo
TANAKA, Francisco Andre Ossamu
AZEVEDO, Joao Lucio
ALMEIDA, Marcilio de
Institución
Resumen
Observations of cells of axenic peach palm (Bactris gasipaes) microplants by light microscopy revealed movements of small particles within the cells. The phenomenon was characterized initially as Brownian movement, but electron microscopy revealed the presence of an intracellular bacterial community in these plants. Microscopy observations revealed the particular shapes of bacterial cells colonizing inner tissues of analyzed plants. Applying a molecular characterization by polymerase chain reaction and denaturing gradient gel electrophoresis, it was revealed the existence of bacterial rRNA within the plants. Sequencing of the rRNA identified three different phylogenetic groups; two bands had a high degree of similarity to sequences from Moraxella sp. and Brevibacillus sp., and a third sequence was similar to a non-cultivated cyanobacterium. The presence of those endosymbionts, called bacteriosomes, in axenic peach palm microplants raises the question of whether these stable endosymbionts were acquired in the process of evolution and how could they benefit the process of plants micropropagation.