Artículos de revistas
Cellular Renewal and Improvement of Local Cell Effector Activity in Peritoneal Cavity in Response to Infectious Stimuli
Fecha
2011Registro en:
PLOS ONE, v.6, n.7, 2011
1932-6203
10.1371/journal.pone.0022141
Autor
CASSADO, Alexandra dos Anjos
ALBUQUERQUE, Jose Antonio Tavares de
SARDINHA, Luiz Roberto
BUZZO, Carina de Lima
FAUSTINO, Lucas
NASCIMENTO, Rogerio
GHOSN, Eliver Eid Bou
LIMA, Maria Regina D'Imperio
ALVAREZ, Jose Maria Mosig
BORTOLUCI, Karina Ramalho
Institución
Resumen
The peritoneal cavity (PerC) is a singular compartment where many cell populations reside and interact. Despite the widely adopted experimental approach of intraperitoneal (i.p.) inoculation, little is known about the behavior of the different cell populations within the PerC. To evaluate the dynamics of peritoneal macrophage (Mempty set) subsets, namely small peritoneal Mempty set (SPM) and large peritoneal Mempty set (LPM), in response to infectious stimuli, C57BL/6 mice were injected i.p. with zymosan or Trypanosoma cruzi. These conditions resulted in the marked modification of the PerC myelo-monocytic compartment characterized by the disappearance of LPM and the accumulation of SPM and monocytes. In parallel, adherent cells isolated from stimulated PerC displayed reduced staining for beta-galactosidase, a biomarker for senescence. Further, the adherent cells showed increased nitric oxide (NO) and higher frequency of IL-12-producing cells in response to subsequent LPS and IFN-gamma stimulation. Among myelo-monocytic cells, SPM rather than LPM or monocytes, appear to be the central effectors of the activated PerC; they display higher phagocytic activity and are the main source of IL-12. Thus, our data provide a first demonstration of the consequences of the dynamics between peritoneal Mempty set subpopulations by showing that substitution of LPM by a robust SPM and monocytes in response to infectious stimuli greatly improves PerC effector activity.