dc.creatorCOLONELLO-FRATTINI, Ninive Aguiar
dc.creatorHARTFELDER, Klaus
dc.date.accessioned2012-04-18T21:54:01Z
dc.date.accessioned2018-07-04T14:35:20Z
dc.date.available2012-04-18T21:54:01Z
dc.date.available2018-07-04T14:35:20Z
dc.date.created2012-04-18T21:54:01Z
dc.date.issued2009
dc.identifierAPIDOLOGIE, v.40, n.4, p.481-495, 2009
dc.identifier0044-8435
dc.identifierhttp://producao.usp.br/handle/BDPI/15264
dc.identifier10.1051/apido/2009009
dc.identifierhttp://dx.doi.org/10.1051/apido/2009009
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1612104
dc.description.abstractThe mating sign that each drone leaves when mating with a queen essentially consists of mucus gland proteins. We employed a Representational Difference Analysis (RDA) methodology to identify genes that are differentially expressed in mucus glands during sexual maturation of drones. The RDA library for mucus glands of newly emerged drones was more complex than that of 8 day-old drones, with matches to 20 predicted genes. Another 26 reads matched to the Apis genome but not to any predicted gene. Since these ESTs were located within ORFs they may represent novel honey bee genes, possibly fast evolving mucus gland proteins. In the RDA library for mucus glands of 8 day-old drones, most reads corresponded to a capsid protein of deformed wing virus, indicating high viral loads in these glands. The expression of two genes encoding venom allergens, acid phosphatase-1 and hyaluronidase, in drone mucus glands argues for their homology with the female venom glands, both associated with the reproductive system.
dc.languageeng
dc.publisherEDP SCIENCES S A
dc.relationApidologie
dc.rightsCopyright EDP SCIENCES S A
dc.rightsopenAccess
dc.subjectmale accessory gland
dc.subjectRepresentational Difference Analysis
dc.subjecthoneybee
dc.subjectreproduction
dc.subjectdeformed wing virus
dc.titleDifferential gene expression profiling in mucus glands of honey bee (Apis mellifera) drones during sexual maturation
dc.typeArtículos de revistas


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