dc.creator | FERREIRA-MELO, Silvia Elaine | |
dc.creator | DEMACQ, Caroline | |
dc.creator | LACCHINI, Silvia | |
dc.creator | KRIEGER, José Eduardo | |
dc.creator | IRIGOYEN, Maria Cláudia | |
dc.creator | MORENO, Heitor | |
dc.date.accessioned | 2012-03-26T21:25:06Z | |
dc.date.accessioned | 2018-07-04T14:23:49Z | |
dc.date.available | 2012-03-26T21:25:06Z | |
dc.date.available | 2018-07-04T14:23:49Z | |
dc.date.created | 2012-03-26T21:25:06Z | |
dc.date.issued | 2011 | |
dc.identifier | Clinics, v.66, n.7, p.1253-1258, 2011 | |
dc.identifier | 1807-5932 | |
dc.identifier | http://producao.usp.br/handle/BDPI/11710 | |
dc.identifier | 10.1590/S1807-59322011000700022 | |
dc.identifier | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322011000700022 | |
dc.identifier | http://www.scielo.br/pdf/clin/v66n7/v66n7a22.pdf | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1609502 | |
dc.description.abstract | OBJECTIVES: We investigated the influence of sildenafil on cardiac contractility and diastolic relaxation and examined the distribution of phosphodiesterase-5 in the hearts of hypertensive rats that were treated with by NG-nitro-L-arginine methyl ester (L-NAME). METHODS: Male Wistar rats were treated with L-NAME and/or sildenafil for eight weeks. The Langendorff method was used to examine the effects of sildenafil on cardiac contractility and diastolic relaxation. The presence and location of phosphodiesterase-5 and phosphodiesterase-3 were assessed by immunohistochemistry, and cGMP plasma levels were measured by ELISA. RESULTS: In isolated hearts, sildenafil prevented the reduction of diastolic relaxation (dP/dt) that was induced by L-NAME. In addition, phosphodiesterase-5 immunoreactivity was localized in the intercalated discs between the myocardial cells. The staining intensity was reduced by L-NAME, and sildenafil treatment abolished this reduction. Consistent with these results, the plasma levels of cGMP were decreased in the L-NAME-treated rats but not in rats that were treated with L-NAME + sildenafil. CONCLUSION: The sildenafil-induced attenuation of the deleterious hemodynamic and cardiac morphological effects of L-NAME in cardiac myocytes is mediated (at least in part) by the inhibition of phosphodiesterase-5. | |
dc.language | eng | |
dc.publisher | Faculdade de Medicina / USP | |
dc.relation | Clinics | |
dc.rights | Copyright Faculdade de Medicina / USP | |
dc.rights | openAccess | |
dc.subject | Hypertension | |
dc.subject | Phosphodiesterase-5 Inhibitor | |
dc.subject | Immunohistochemistry | |
dc.title | Sildenafil preserves diastolic relaxation after reduction by L-NAME and increases phosphodiesterase-5 in the intercalated discs of cardiac myocytes and arterioles | |
dc.type | Artículos de revistas | |