dc.creatorRUDI EMERSON DE LIMA, Procópio
dc.creatorARAÚJO, Welington Luiz
dc.creatorANDREOTE, Fernando Dini
dc.creatorAZEVEDO, João Lúcio
dc.date.accessioned2012-03-26T02:38:59Z
dc.date.accessioned2018-07-04T13:55:25Z
dc.date.available2012-03-26T02:38:59Z
dc.date.available2018-07-04T13:55:25Z
dc.date.created2012-03-26T02:38:59Z
dc.date.issued2011
dc.identifierGenetics and Molecular Biology, v.34, n.1, p.103-109, 2011
dc.identifier1415-4757
dc.identifierhttp://producao.usp.br/handle/BDPI/4705
dc.identifier10.1590/S1415-47572010005000096
dc.identifierhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100018
dc.identifierhttp://www.scielo.br/pdf/gmb/v34n1/2010-227.pdf
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1603503
dc.description.abstractA circular cryptic plasmid named pPAGA (2,734 bp) was isolated from Pantoea agglomerans strain EGE6 (an endophytic bacterial isolate from eucalyptus). Sequence analysis revealed that the plasmid has a G+C content of 51% and contains four potential ORFs, 238(A), 250(B), 131(C), and 129(D) amino acids in length without homology to known proteins. The shuttle vector pLGM1 was constructed by combining the pPAGA plasmid with pGFPmut3.0 (which harbors a gene encoding green fluorescent protein, GFP), and the resulting construct was used to over-express GFP in E. coli and P. agglomerans cells. GFP production was used to monitor the colonization of strain EGE6gfp in various plant tissues by fluorescence microscopy. Analysis of EGE6gfp colonization showed that 14 days after inoculation, the strain occupied the inner tissue of Eucalyptus grandis roots, preferentially colonizing the xylem vessels of the host plants.
dc.languageeng
dc.publisherSociedade Brasileira de Genética
dc.relationGenetics and Molecular Biology
dc.rightsCopyright Sociedade Brasileira de Genética
dc.rightsopenAccess
dc.subjectGreen fluorescent protein
dc.subjectBacteria
dc.subjectHost plant
dc.subjectEucalyptus
dc.titleCharacterization of a small cryptic plasmid from endophytic Pantoea agglomerans and its use in the construction of an expression vector
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución