Artículos de revistas
PCR detection of canine distemper virus in biological fluids
Fecha
2008Registro en:
Online Journal of Veterinary Research, [S.l.], v. 12, n. 2, p. 70-75, 2008
1328-925X
Autor
AMARAL, H. A
CORTEZ, A
RICHTZENHAIN, Leonardo José
FUNADA, Mikaela Renata
SOARES, Rodrigo Martins
DURIGON, Edison Luiz
LARSSON, Maria Helena Matiko Akao
Institución
Resumen
Canine distemper is a highly contagious disease affecting dogs worldwide, often inducing severe neurological signs. The aim of this study was to evaluate different biological samples (conjunctival and genital swabs, urine sample and peripheral blood mononuclear cells) by reverse-transcription hemi-nested polymerase chain reaction (PCR). All collections were taken from dogs with neurological manifestations, examined at the Veterinary Hospital at the School of Veterinary Medicine and Animal Science, University of São Paulo, from October 2004 to May 2007. Only dogs that had not been vaccinated or that were vaccinated more than 50 days before the samples were collected were included to exclude any false-positive results from vaccine virus. Fragments of nucleoprotein genes canine distemper virus were detected by hemi-nested PCR in 43 (86%) of the 50 dogs included in the study. A larger number of positive results were obtained from genital swabs with 40 (80%) positive samples, followed by 37 (74%) from conjunctival swabs, 37 (74%) from urine samples and 33 (68%) peripheral blood mononuclear cells. Conjunctival and genital swabs, and urine samples proved to be excellent choices of clinical sample because of their high detection sensitivity, convenient sample processing and less risk to the animals. Sensitivity in detecting positive results was increased by testing two clinical samples (genital or conjunctival swab and urine sample), especially in dogs that had not shown extra-neural signs or those in the convalescent and late stage of canine distemper