Survey of feline leukemia virus and feline coronaviruses in captive neotropical wild felids from southern Brazil

dc.creatorGUIMARÃES, A. M. S.
dc.creatorBRANDÃO, Paulo E.
dc.creatorMORAES, Wanderlei de
dc.creatorCUBAS, Zalmir Silvino
dc.creatorSANTOS, Leonilda Correia dos
dc.creatorVILLARREAL BUITRAGO, Laura Yaneth
dc.creatorROBES, R. R
dc.creatorCOELHO, F. M
dc.creatorRESENDE, Maurício
dc.creatorSANTOS, R. C. F
dc.creatorOLIVEIRA, R. C
dc.creatorYAMAGUTI, Maurício
dc.creatorMARQUES, L. M
dc.creatorLOPES NETO, Renata
dc.creatorBUZINHANI, Melissa
dc.creatorMARQUES, Regina
dc.creatorMESSICK, J. B
dc.creatorBIONDO, Alexander Welker
dc.creatorTIMENETSKY, Jorge
dc.date.accessioned2012-03-19T23:16:16Z
dc.date.accessioned2018-07-04T13:44:54Z
dc.date.available2012-03-19T23:16:16Z
dc.date.available2018-07-04T13:44:54Z
dc.date.created2012-03-19T23:16:16Z
dc.date.issued2009
dc.identifierJournal of Zoo and Wildlife Medicine, Lawrence, v. 40, n. 2, p. 360-364, 2009
dc.identifier1042-7260
dc.identifierhttp://producao.usp.br/handle/BDPI/2000
dc.identifierhttp://www.bioone.org/doi/pdf/10.1638/2008-0067.1
dc.identifier1937-2825
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1601324
dc.description.abstractAbstract: A total of 57 captive neotropical felids (one Leopardus geoffroyi, 14 Leopardus pardalis, 17 Leopardus wiedii, 22 Leopardus tigrinus, and three Puma yagouaroundi) from the Itaipu Binacional Wildlife Research Center (Refu´gio Bela Vista, Southern Brazil) were anesthetized for blood collection. Feces samples were available for 44 animals, including one L. geoffroyi, eight L. pardalis, 14 L. wiedii, 20 L. tigrinus, and one P. yagouaroundi. Total DNA and RNA were extracted from blood and feces, respectively, using commercial kits. Blood DNA samples were evaluated by polymerase chain reaction (PCR) for feline leukemia virus (FeLV) proviral DNA, whereas reverse transcriptase–PCR was run on fecal samples for detection of coronavirus RNA. None of the samples were positive for coronaviruses. A male L. pardalis and a female L. tigrinus were positive for FeLV proviral DNA, and identities of PCR products were confirmed by sequencing. This is the first evidence of FeLV proviral DNA in these species in Southern Brazil.
dc.languageen
dc.publisherLawrence
dc.relationJournal of Zoo and Wildlife Medicine
dc.rightsopenAccess
dc.subjectFeline coronavirus
dc.subjectfeline leukemia virus
dc.subjectLeopardus pardalis
dc.subjectLeopardus tigrinus
dc.subjectneotropical cats
dc.titleSurvey of feline leukemia virus and feline coronaviruses in captive neotropical wild felids from southern Brazil
dc.typeArtículos de revistas


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