| dc.contributor | Velezmoro Sánchez, Carmen Eloisa | |
| dc.creator | Casas Vásquez, José Paúl | |
| dc.date | 2017-08-01T20:01:36Z | |
| dc.date | 2017-08-01T20:01:36Z | |
| dc.date | 2016 | |
| dc.date.accessioned | 2018-04-02T19:41:48Z | |
| dc.date.available | 2018-04-02T19:41:48Z | |
| dc.identifier | Q03.C383-T BAN UNALM | |
| dc.identifier | http://repositorio.lamolina.edu.pe/handle/UNALM/2712 | |
| dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1370129 | |
| dc.description | Universidad Nacional Agraria La Molina. Escuela de Posgrado. Maestría en Tecnología de Alimentos | |
| dc.description | La presente investigación tuvo como objetivo el diseño de un software con base en el método PEIE (Paired Equivalent Isothermal Exposure), un modelo matemático utilizado para determinar parámetros cinéticos de la destrucción de esporas bacterianas; Bacillus subtilis/amyloliquefaciens, en modo no isotérmico. Para las pruebas de termorresistencia se emplearon discos de aluminio TDT (Thermal Death Time). En cada disco TDT se colocó 1.00 mL de muestra inoculada, aproximadamente 0.9 mL de puré de conservas de espárragos blancos (pH = 4.9) y 0.1 mL de esporas en suspensión. Tanto la concentración inicial y final de microorganismos como los perfiles de temperatura son datos de entrada para el funcionamiento del software, desarrollado en lenguaje Visual Basic. Los parámetros cinéticos de destrucción térmica del Bacillus subtilis/amyloliquefaciens encontrados con el software KineticLab a temperaturas inferiores a 100°C fueron: Ea = 180.454 ± 4.68 kJ/mol, valores D (D85°C = 16.85 min ± 0.67, D90°C = 7.39 min ± 0.24 y D95°C = 3.24 min ± 0.12) y para temperaturas superiores a 100°C fueron: Ea = 218.068 ± 5.61 kJ/mol, valores D (D100°C = 1.38 min ± 0.23, D110°C = 0.22 min ± 0.03 y D120°C = 0.037 min ± 0.01). | |
| dc.description | The objetive of this research was to develop a software based on the PEIE method (Paired Equivalent Isothermal Exposure) which is a non-isothermal mathematical model to determine kinetic parameters for the destruction of bacterial spores: Bacillus subtilis / amyloliquefaciens. Aluminum TDT (Thermal Death Time) disks were used for thermal resistance tests. In each TDT disk was placed 1.00 mL of inoculated sample, about 0.9 mL of canned white asparagus puree (pH = 4.9) and 0.1 mL of spores in suspension. Both the initial and final concentration of microorganisms and the temperature profiles were input for the software operation which was developed in Visual Basic language. Kinetic parameters of the Bacillus subtilis/ amyloliquefaciens thermal destruction obtained by KineticLab software at temperatures below 100°C were Ea = 180.454 ± 4.68 kJ/mol, and D value (D85°C = 16.85 min ± 0.67, D90°C = 7.39 min ± 0.24 y D95°C = 3.24 min ± 0.12) and at temperatures over 100°C were: Ea = 218.068 ± 5.61 kJ/mol, and values D (D100°C = 1.38 min ± 0.23, D110°C = 0.22 min ± 0.03 y D120°C = 0.037 min ± 0.01). | |
| dc.description | Tesis | |
| dc.format | application/pdf | |
| dc.language | spa | |
| dc.publisher | Universidad Nacional Agraria La Molina | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.rights | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
| dc.source | Universidad Nacional Agraria La Molina | |
| dc.source | Repositorio institucional - UNALM | |
| dc.subject | Asparagus officinalis | |
| dc.subject | Bacillus subtilis | |
| dc.subject | Esporas | |
| dc.subject | Cinesis | |
| dc.subject | Tratamiento térmico | |
| dc.subject | Programas de ordenador | |
| dc.subject | Evaluación | |
| dc.subject | Perú | |
| dc.subject | Parámetros cinéticos | |
| dc.subject | Bacillus subtilis/amyloliquefaciens | |
| dc.subject | Método no isotérmico | |
| dc.subject | Software kinetic lab | |
| dc.title | Software para la determinación de parámetros cinéticos de la destrucción de esporas Bacillus subtilis/amyloliquefaciens por un método no isotérmico | |
| dc.type | Tesis | |
