Development Of A Water-in-oil-in-water Multiple Emulsion System Integrating Biomimetic Aqueous-core Lipid Nanodroplets For Protein Entity Stabilization. Part Ii: Process And Product Characterization

dc.creatorGlasser
dc.creatorCassia A.; Vila
dc.creatorMarta M. D. C.; Pereira
dc.creatorJulio C.; Chaud
dc.creatorMarco V.; Oliveira Junior
dc.creatorJose M.; Tubino
dc.creatorMatthieu; Balcao
dc.creatorVictor M.
dc.date2016
dc.datedez
dc.date2017-11-13T13:56:55Z
dc.date2017-11-13T13:56:55Z
dc.date.accessioned2018-03-29T06:10:14Z
dc.date.available2018-03-29T06:10:14Z
dc.identifierDrug Development And Industrial Pharmacy . Taylor & Francis Ltd, v. 42, p. 1990 - 2000, 2016.
dc.identifier0363-9045
dc.identifier1520-5762
dc.identifierWOS:000385567100008
dc.identifier10.1080/03639045.2016.1188109
dc.identifierhttp://www.tandfonline.com/doi/abs/10.1080/03639045.2016.1188109
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/329963
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1366988
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionThe aqueous-core enclosed in lipid nanoballoons integrating multiple emulsions of the type water-in-oil-in-water mimic, at least in theory, the environment within viable cells, thus being suitable for housing hydrophilic protein entities such as bioactive proteins, peptides and bacteriophage particles. This study reports a complete physicochemical characterization of optimized biomimetic aqueous-core lipid nanoballoons housing hydrophilic (BSA) protein entities, evolved from a statistical 2(3)x3(1) factorial design study (three variables at two levels and one variable at three levels) that was the subject of the first paper of a series of three, aiming at complete stabilization of the three-dimensional structure of protein entities attempted via housing the said molecular entities within biomimetic aqueous-core lipid nanoballoons integrating a multiple (W/O/W) emulsion. The statistical factorial design followed led to the production of an optimum W/O/W multiple emulsion possessing quite homogeneous particles with an average hydrodynamic size of (186.2 +/- 2.6) nm and average Zeta potential of (-36.5 +/- 0.9) mV, and exhibiting a polydispersity index of 0.206 +/- 0.014. Additionally, the results obtained for the diffusion coefficient of the lipid nanoballoons integrating the optimized W/O/W multiple emulsion were comparable and of the same order of magnitude (10(-12) m(2)s(-1)) as those published by other authors since, typically, diffusion coefficients for molecules range from 10(-10) to 10(-7) m(2)s(-1), but diffusion coefficients for nanoparticles are typically of the order of magnitude of 10(-12) m(2)s(-1).
dc.description42
dc.description12
dc.description1990
dc.description2000
dc.descriptionCNPq, National Council for Scientific and Technological Development - Brazil [306113/2014-7, 309598/2014-1]
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageEnglish
dc.publisherTaylor & Francis Ltd
dc.publisherAbingdon
dc.relationDrug Development and Industrial Pharmacy
dc.rightsfechado
dc.sourceWOS
dc.subjectAqueous-core Lipid Nanoballoons
dc.subjectDiffusion Coefficient
dc.subjectInfrared Spectrophotometry With Fourier Transform
dc.subjectProtein Entities
dc.subjectStokes-einstein Equation
dc.subjectStructural And Functional Stabilization
dc.subjectWater-in-oil-in-water Multiple Emulsions
dc.subjectX-ray Diffraction Analysis
dc.titleDevelopment Of A Water-in-oil-in-water Multiple Emulsion System Integrating Biomimetic Aqueous-core Lipid Nanodroplets For Protein Entity Stabilization. Part Ii: Process And Product Characterization
dc.typeArtículos de revistas


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