Hypothalamic Stearoyl-coa Desaturase-2 (scd2) Controls Whole-body Energy Expenditure

dc.creatorde Moura
dc.creatorR. F.; Nascimento
dc.creatorL. F.; Ignacio-Souza
dc.creatorL. M.; Morari
dc.creatorJ.; Razolli
dc.creatorD. S.; Solon
dc.creatorC.; de Souza
dc.creatorG. F. P.; Festuccia
dc.creatorW. T.; Velloso
dc.creatorL. A.
dc.date2016
dc.datemar
dc.date2017-11-13T13:56:34Z
dc.date2017-11-13T13:56:34Z
dc.date.accessioned2018-03-29T06:09:54Z
dc.date.available2018-03-29T06:09:54Z
dc.identifierInternational Journal Of Obesity. Nature Publishing Group, v. 40, p. 471 - 478, 2016.
dc.identifier0307-0565
dc.identifier1476-5497
dc.identifierWOS:000371799300011
dc.identifier10.1038/ijo.2015.188
dc.identifierhttp://www.nature.com/ijo/journal/v40/n3/full/ijo2015188a.html
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/329887
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1366912
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionStearoyl-CoA desaturase-2 (SCD2) is the main delta 9 desaturase expressed in the central nervous system. Because of its potential involvement in controlling whole-body adiposity, we evaluated the expression and function of SCD2 in the hypothalami of mice. SUBJECTS/METHODS: Male mice of different strains were used in real-time PCR, immunoblot and metabolic experiments. In addition, antisense oligonucleotides and lentiviral vectors were used to reduce and increase the expression of SCD2 in the hypothalamus. RESULTS: The level of SCD2 in the hypothalamus is similar to other regions of the central nervous system and is similar to 10-fold higher than in any other region of the body. In the arcuate nucleus, SCD2 is expressed in proopiomelanocortin and neuropeptide-Y neurons. Upon high fat feeding, the level of hypothalamic SCD2 increases. Inhibition of hypothalamic SCD2 as accomplished by two distinct approaches, an antisense oligonucleotide or a short-hairpin RNA delivered by a lentivirus, resulted in reduced body mass gain mostly due to increased energy expenditure and increased spontaneous activity. Increasing hypothalamic SCD2 by a lentivirus approach resulted in no change in body mass and food intake. CONCLUSIONS: Thus, SCD2 is highly expressed in the hypothalami of rodents and its knockdown reduces body mass due to increased whole-body energy expenditure.
dc.description40
dc.description3
dc.description471
dc.description478
dc.descriptionSao Paulo Research Foundation
dc.descriptionConselho Nacional de Desenvolvimento Cientifico e Tecnologico
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageEnglish
dc.publisherNature Publishing Group
dc.publisherLondon
dc.relationInternational Journal of Obesity
dc.rightsfechado
dc.sourceWOS
dc.subjectInsulin-resistance;gene-expression;mediterranean Diet;obesity;fat;activation;inflammation;prevention;coenzyme;rodents
dc.titleHypothalamic Stearoyl-coa Desaturase-2 (scd2) Controls Whole-body Energy Expenditure
dc.typeArtículos de revistas


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