This study examined the physiological responses during exercise-to-exhaustion at the lactate-minimum-intensity with and without prior high-intensity exercise. Eleven recreationally trained males performed a graded exercise test, a lactate minimum test and two constant-load tests at lactate-minimum-intensity until exhaustion, which were applied with or without prior hyperlactatemia induction (i.e., 30-s Wingate test). The physiological responses were significantly different (P<0.05) between constant-load tests for pulmonary ventilation (V-E), blood-lactate-concentration ([La-]), pH, bicarbonate concentration ([HCO3]) and partial pressure of carbon dioxide during the initial minutes. The comparisons within constant-load tests showed steady state behaviour for oxygen uptake and the respiratory exchange ratio, but heart rate and rating of perceived exertion increased significantly during both exercise conditions, while the V-E increased only during constant-load effort. During effort performed after high-intensity exercise: V-E, [La-], pH and [HCO3] differed at the start of exercise compared to another condition but were similar at the end (P>0.05). In conclusion, the constant-load exercises performed at lactate-minimum-intensity with or without prior high-intensity exercise did not lead to the steady state of all analysed parameters; however, variables such as [La-], pH and [HCO3] - altered at the beginning of effort performed after high-intensity exercise - were reestablished after approximately 30 min of exercise.342121062113