Actas de congresos
[des-arg(1)]-proctolin: A Novel Nep-like Enzyme Inhibitor Identified In Tityus Serrulatus Venom
Registro en:
Peptides . Elsevier Science Inc , v. 80, p. 18 - 24, 2016.
0196-9781
1873-5169
WOS:000375936400004
10.1016/j.peptides.2015.05.013
Autor
Duzzi
Bruno; Cajado-Caryalho
Daniela; Kuniyoshi
Alexandre Kazuo; Kodama
Roberto Tadashi; Gozzo
Fabio Cesar; Fioramonte
Mariana; Tambourgi
Denise Vilarinho; Portaro
Fernanda Vieira; Rioli
Vanessa
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) The scorpion Tityus serrulatus venom comprises a complex mixture of molecules that paralyzes and kills preys, especially insects. However, venom components also interact with molecules in humans, causing clinic envenomation. This cross-interaction may result from homologous molecular targets in mammalians and insects, such as (NEP)-like enzymes. In face of these similarities, we searched for peptides in Tityus serrulatus venom using human NEP as a screening tool. We found a NEP-inhibiting peptide with the primary sequence YLPT, which is very similar to that of the insect neuropeptide proctolin (RYLPT). Thus, we named the new peptide [des-Arg(1)]-proctolin. Comparative NEP activity assays using natural substrates demonstrated that [des-Arg(1)]-proctolin has high specificity for NEP and better inhibitory activity than proctolin. To test the initial hypothesis that molecular homologies allow Tityus serrulatus venom to act on both mammal and insect targets, we investigated the presence of a NEP-like in cockroaches, the main scorpion prey, that could be likewise inhibited by [des-Arg(1)]-proctolin. Indeed, we detected a possible NEP-like in a homogenate of cockroach heads whose activity was blocked by thiorphan and also by [des-Arg(1)]-proctolin. Western blot analysis using a human NEP monoclonal antibody suggested a NEP-like enzyme in the homogenate of cockroach heads. Our study describes for the first time a proctolin-like peptide, named [des-Arg(1)]-proctolin, isolated from Tityus serrulatus venom. The tetrapeptide inhibits human NEP activity and a NEP-like activity in a cockroach head homogenate, thus it may play a role in human envenomation as well as in the paralysis and death of scorpion preys. (C) 2015 Elsevier Inc. All rights reserved. 80 18 24 Fundacao de Amparo a Pesquisa do Estado de Sao Paulo [2014/12976-5, 2012/06677-0, 2013/15343-0] Instituto Nacional de Ciencia e Tecnologia em Toxinas-Conselho Nacional de Desenvolvimento Cientifico e Tecnologico Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Invertebrate Neuropeptide Conference 2015 (INC2015). 2015 Bagan, MYANMAR